LC-MS Klinisk Kemi Region Skåne Anders Blomgren Utbildningsdagar, Saltsjöbaden 8:e feb 2017
Innehåll Region Skåne, SUS, Klinisk Kemi LC-MS verksamheten - Lokaler/Instrumentering/Arbetssätt - Analyserna
Klinisk Kemi, Region Skåne Universitetssjukhus Akutsjukhus Närsjukhus
Klinisk Kemi, Region Skåne
Klinisk Kemi, Region Skåne LC-MS system på Klin Kem före 2015-2 i Lund, 2 i Malmö och 1 i Helsingborg 2013 - Samlokalisering Masspektrometri - kritisk massa - samlad kompetensen - åldersavgångar - bättre utnyttjande av instrumenten Ombyggnation befintligt och nytt MS-lab Sista systemet flyttat jan 2016
Ombyggnation MS lab Media Högt ljud Värme/Kyla/Drag Serviceåtkomst Visuell kontakt
Nytt lab Ombyggnation MS lab Ombyggt lab Enhetligt (mjukvara, instrument) Tidsplan för utbyte
Provupparbetning
Provupparbetning Robotar - är snabba - ger bra precision - gör likadant varje gång - blir inte uttråkade - är enkla att reparera Metoder - proteinfällning så länge det går - fällningsplatta - SPE (3 robotar utrustade) - (2D-LC)
Personal Anders Blomgren Kemister, 5 st Läkemedel Missbruk Endokrinologi Protein Robot (ny) Therese Hansson Benny Larsson Per Bengtsson Maria Fröjd
Analyserna S-MMA S-Hepcidin Utveckling Utbildning Läkemedel U/P-Metoxykatekolaminer U-Kortisol Steroider (Östradiol Androstendion Testosteron 17OH-Progesteron) B-PEth S-Metformin S-Vit D
XIC of +MRM (22 pairs): 315.200/58.200 amu Expected RT: 3.0 ID: S-Klomi from Sample 8 (Antidep kont hög) of ADEPR_160629_78A.wi... Max. 4.8e6 cps. 4.8e6 4.6e6 4.4e6 4.2e6 4.0e6 3.8e6 3.6e6 3.4e6 3.2e6 3.0e6 2.8e6 2.6e6 2.4e6 2.2e6 2.0e6 1.8e6 1.6e6 1.4e6 1.2e6 1.0e6 8.0e5 6.0e5 4.0e5 Biphenyl 2.0e5 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 Antidepressiva 2.99 XIC of +MRM (5 pairs): 975.400/908.500 Da ID: B-Evero from Sample 8 (immuno kont hög) of IMMUN_160705_37A.wiff (Turbo Spray) Max. 5.1e5 cps. 9.3e5 9.0e5 8.5e5 8.0e5 7.5e5 7.0e5 6.5e5 6.0e5 5.5e5 1.70 5.0e5 4.5e5 4.0e5 3.5e5 3.0e5 2.5e5 2.0e5 1.5e5 1.0e5 5.0e4 Biphenyl 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 Immunosuppressiva XIC of +MRM (10 pairs): 438.200/171.200 Da ID: Flufenazin from Sample 8 (LCMS1 kont hög) of LCMS1_160704_25A.wiff (Turbo Spray... Max. 3.7e6 cps. XIC of +MRM (16 pairs): 411.200/191.100 amu Expected RT: 1.6 ID: S-Rispe from Sample 8 (LCMS2 kon hög) of LCMS2_160704_29A.w... Max. 4.0e6 cps. 8.3e6 8.0e6 7.5e6 7.0e6 6.5e6 6.0e6 5.5e6 5.0e6 4.5e6 4.0e6 1.46 3.5e6 3.0e6 2.5e6 2.0e6 1.5e6 1.0e6 5.0e5 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2.0 2.1 2.2 2.3 Neuroleptika 1 Biphenyl 8.0e6 7.5e6 7.0e6 6.5e6 6.0e6 5.5e6 5.0e6 4.5e6 4.0e6 3.5e6 3.0e6 2.5e6 2.0e6 1.5e6 1.0e6 5.0e5 Biphenyl 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 Neuroleptika 2 1.57
XIC of +MRM (14 pairs): 645.800/58.000 amu Expected RT: 3.8 ID: Amiod-S from Sample 8 (LCMS4 Kon Hö) of LCMS4_160629_27A.wif... Max. 2.9e6 cps. XIC of +MRM (22 pairs): 389.100/245.100 amu Expected RT: 0.9 ID: Zopik-S from Sample 8 (LCMS6 kon hö) of LCMS6_160630_02A.wi... Max. 2.4e6 cps. 3.9e6 3.8e6 3.6e6 5.3e6 5.0e6 3.4e6 3.2e6 4.5e6 3.0e6 2.8e6 3.63 4.0e6 2.6e6 3.5e6 2.4e6 2.2e6 2.0e6 1.8e6 1.6e6 3.0e6 2.5e6 1.00 1.4e6 2.0e6 1.2e6 1.0e6 1.5e6 8.0e5 6.0e5 1.0e6 4.0e5 2.0e5 Biphenyl 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 Antiarytmika 5.0e5 Biphenyl 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Bensodiazipiner XIC of -MRM (9 pairs): 701.400/255.200 Da ID: Peth 16:0 18:1_255 from Sample 10 (PEth kont Hög) of LCMS8_160706_68A.wiff (Turbo... Max. 1.0e6 cps. XIC of +MRM (8 pairs): 219.190/162.100 amu Expected RT: 1.1 ID: Primidon from Sample 8 (LCMS7 kon hö) of LCMS7_160705_27A.wiff... 3.1e6 3.0e6 1.08 2.8e6 2.6e6 2.4e6 2.2e6 Max. 3.0e6 cps. 2.3e6 2.2e6 2.1e6 2.0e6 1.9e6 1.8e6 1.7e6 1.6e6 1.5e6 2.0e6 1.8e6 1.6e6 1.4e6 1.4e6 1.3e6 1.2e6 1.1e6 1.0e6 9.0e5 1.25 1.2e6 8.0e5 1.0e6 7.0e5 6.0e5 8.0e5 5.0e5 6.0e5 4.0e5 2.0e5 Biphenyl 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Antiepileptika 4.0e5 3.0e5 2.0e5 1.0e5 1.42 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 B-PEth 16:0/18:1 C18
XIC of +MRM (6 pairs): 166.075/134.200 Da ID: U-MNA from Sample 5 (METOX kon hö) of METOX_160704_13A.wiff (Turbo Spray) Max. 1.0e6 cps. XIC of +MRM (6 pairs): 166.075/134.200 Da ID: P-MNA from Sample 1 (Sample001) of Kal 3 10 ul 161130SET1.wiff (Turbo Spray) Max. 2.0e4 cps. XIC of -MRM (8 pairs): 125.000/97.000 Da ID: EtS quant from Sample 8 (Hk) of 160111_BlankU_sp_vatten.wiff (Turbo Spray) Max. 7.8e6 cps. 1.04e6 1.00e6 9.50e5 9.00e5 8.50e5 8.00e5 7.50e5 7.00e5 6.50e5 6.00e5 5.50e5 5.00e5 4.50e5 4.00e5 3.50e5 3.00e5 2.50e5 2.00e5 1.50e5 1.00e5 5.00e4 Lång C18 2.08 0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 U-Metoxikatekolaminer 3.2e5 3.0e5 2.8e5 2.6e5 2.4e5 2.2e5 2.0e5 1.8e5 1.6e5 1.4e5 1.2e5 1.0e5 8.0e4 6.0e4 4.0e4 2.0e4 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 4.43 HILIC P-Metoxikatekolaminer 7.8e6 7.5e6 7.0e6 6.5e6 6.0e6 5.5e6 5.0e6 4.5e6 4.0e6 3.5e6 3.0e6 2.5e6 2.0e6 1.5e6 1.0e6 5.0e5 2.18 Hydrofil C18 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 EtG/EtS XIC of +MRM (12 pairs): 130.100/70.800 Da from Sample 8 (Metformin Kon Hög) of METFO_160414_20A.wiff (Turbo Spray) Max. 4.5e5 cps. XIC of -MRM (2 pairs): 116.800/72.800 Da ID: MMA from Sample 6 (MMA kon hög) of MMA_160705_52A.wiff (Turbo Spray IonDrive) Max. 2.3e5 cps. XIC of +MRM (2 pairs): 558.800/693.800 Da ID: Hep 559/694 from Sample 9 (HEPCI kon hög) of HEPC_160706_36A.wiff (Turbo Spray I... Max. 1.7e4 cps. 4.4e5 4.2e5 4.0e5 3.8e5 3.6e5 2.69 3.1e5 3.0e5 2.8e5 2.6e5 1.7e4 1.6e4 1.5e4 1.4e4 0.98 3.4e5 2.4e5 0.38 1.3e4 3.2e5 3.0e5 2.8e5 2.6e5 2.4e5 2.2e5 2.0e5 2.2e5 2.0e5 1.8e5 1.6e5 1.4e5 1.2e4 1.1e4 1.0e4 900 800 1.8e5 1.6e5 1.4e5 1.2e5 1.0e5 8.0e4 6.0e4 4.0e4 2.0e4 ZIC-HILIC 1.2e5 1.0e5 8.0e4 6.0e4 4.0e4 2.0e4 0.78 HILIC 700 600 500 400 300 200 100 C18 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Metformin 0 5 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 0.55 0.60 0.65 0.70 0.75 0.80 0.85 0.90 0.95 1.00 1.05 1.10 1.15 MMA 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 Hepcidin
XIC of -MRM (4 pairs): 407.100/331.200 Da ID: Kortisol 331 from Sample 8 (U-kort hög) of STER1_160630_04A.wiff (Turbo Spray) Max. 8.5e5 cps. XIC of +MRM (3 pairs): 292.200/96.800 Da ID: Testosterone d3 from Sample 4 (kal 4) of Testo körning 150121SET1.wiff (Heated Nebuliz... Max. 1.1e5 cps. XIC of -MRM (7 pairs): 359.100/331.100 Da ID: Aldo 2 from Sample 7 (k6) of Aldosteron 141124SET1.wiff (Turbo Spray) Max. 1.4e4 cps. XIC of +MRM (12 pairs): 331.200/97.000 Da ID: 17OHP 1 from Sample 7 (kal 5) of Steroid 121212SET1.wiff (Heated Nebulizer) Max. 2.4e4 cps. 8.5e5 8.0e5 1.62 1.14e5 1.10e5 1.05e5 3.68 3.0e4 2.8e4 2.4e4 2.3e4 2.2e4 3.97 7.5e5 1.00e5 2.6e4 2.1e4 7.0e5 6.5e5 9.50e4 9.00e4 8.50e4 2.4e4 2.2e4 2.0e4 1.9e4 1.8e4 6.0e5 5.5e5 5.0e5 4.5e5 4.0e5 3.5e5 3.0e5 2.5e5 2.0e5 1.5e5 1.0e5 5.0e4 Biphenyl 8.00e4 7.50e4 7.00e4 6.50e4 6.00e4 5.50e4 5.00e4 4.50e4 4.00e4 3.50e4 3.00e4 2.50e4 2.00e4 1.50e4 1.00e4 5000 2D-LC 2.86 2.0e4 1.8e4 1.6e4 1.4e4 1.2e4 1.0e4 800 600 400 200 2.34 2D-LC 1.7e4 1.6e4 1.5e4 1.4e4 1.3e4 1.2e4 1.1e4 1.0e4 900 800 700 600 500 400 300 200 100 2D-LC 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 U-kortisol 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 S-Testosteron* 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 S-Aldosteron* 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 S-17-HO-Progesteron* XIC of +MRM (4 pairs): 383.313/211.200 Da ID: VitD3 1 from Sample 4 (VITD L2) of VITD_160706_69A.wiff (Heated Nebulizer) Max. 3.2e4 cps. XIC of +MRM (11 pairs): 287.200/97.000 Da ID: Androstenedione 1 from Sample 10 (ANDRO Kon Hö) of ANDR_160628_43A.wiff (Heate... Max. 2.2e5 cps. XIC of -MRM (6 pairs): 270.992/143.128 Da ID: Östradiol 1 from Sample 7 (ESTRA Kon Hö) of ESTRA_160630_26A.wiff (Turbo Spray) Max. 2176.0 cps. 6.8e4 6.5e4 2.2e5 2.1e5 4.01 1.7e4 1.6e4 6.0e4 5.5e4 2.0e5 1.9e5 1.8e5 1.7e5 1.5e4 1.4e4 1.3e4 5.0e4 4.5e4 4.0e4 3.5e4 3.0e4 1.39 1.6e5 1.5e5 1.4e5 1.3e5 1.2e5 1.1e5 1.0e5 9.0e4 3.40 1.2e4 1.1e4 1.0e4 900 800 700 2.5e4 8.0e4 600 2.0e4 1.5e4 7.0e4 6.0e4 5.0e4 500 400 1.0e4 500 C18 1.49 4.0e4 3.0e4 2.0e4 1.0e4 2D-LC, C18 3.80 300 200 100 5.91 2D-LC, C8 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 S-Vitamin D 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 S-Androstendion 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 1 10.5 11.0 11.5 12.0 12.5 S-Östradiol * Inte tagen i bruk än
Analyserna S-Transferrin proteoformer Apo1-Lipoprotein 1-antitrypsin Fria lätta kedjor Drugs of abuse Verifiering Drugs of abuse Verifiering (Kannabinoider)
XIC of -MRM (4 pairs): 343.200/299.100 Da ID: THC-COOH 1 from Sample 13 (971231-3056) of DROG1_170104_10A.wiff (Turbo Spray... 2.81 3.6e5 Max. 3.7e5 cps. +41 +40 +39 +38 +37 3.4e5 3.2e5 3.0e5 2.8e5 2.6e5 2.4e5 2.2e5 2.0e5 1.8e5 1.6e5 1.4e5 1.2e5 1.0e5 8.0e4 6.0e4 4.0e4 2.0e4 Biphenyl 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Kannabinoider (THC-COOH) S-Transferrin proteoformer (2D-LC, affinitet & C4) -1-antitrypsin (2D-LC, affinitet & C4)
Fria lätta kedjor (2D-LC, affinitet & C4)
Problem / Hot Personal o Åldersavgångar o Antal IT o Föråldrat o Remisser o Resurser
Tack!