A G M K. Supplemental Figure S1.

G A G M K F I D I G G G L G Supplementl Figure S1.

CADC1 MPALGCCVDATVSPPLGYAFSSDSSLPTPEFFSSGVPPMTNAAAGHSHWSPDLSSALYRVD 61 Tocco ADC MPALGCCVDAAVVSPPLSYAFSRDSSLPAPEFFASGVPPTNSAAASHWSPDLSSALYGVD 6 Aridopsis ADC2 MPALACVDTSFVPPAYAFSDTAGDVFIPASSPTSAAVVVDRWSPSLSSSLYRID 54 Grden pe ADC MPALTCFVDGAAALLHPPGYALAGDFTLPLPFTFSAAATITDDADATAVEDSNSIWSPSLSSKLFRID 68 Aridopsis ADC1 MPALAFVDTPIDTFSSIFTPSSVSTAVVDGSCHWSPSLSSSLYRID 46 CADC1 GWGAPYFSVNSSGDISVRPHGTDTLPHQEIDLLKVAKKAADPKHLGGLGLQMPLVVRFPDILKNRLET 129 Tocco ADC GWGAPYFSVNSNGDISVRPHGTDTLPHQEIDLLKVVKKASDPKNSGGLGLQLPLVVRFPDVLKNRLES 128 Aridopsis ADC2 GWGAPYFIANSSGNISVRPHGSETLPHQDIDLLKIVKKVTGPKSSGGLGLQLPLIVRFPDVLKNRLEC 122 Grden pe ADC GWGFPYFGVNAAGDISVRPHGSATMSHQEIDLLKVVKKASDPKCCGGLGLQLPLVVRFPDVLKDRLES 136 Aridopsis ADC1 GWGAPYFAANSSGNISVRPHGSNTLPHQDIDLMKVVKKVTDPSGLGLQLPLIVRFPDVLKNRLEC 111 ==================== CADC1 LQSAFDMAVRSRGYEAHYQGVYPVKCNQDRFVVEDIVKFGSPYRFGLEAGSKPELLLAMNCLSKGSAD 197 Tocco ADC LQSAFDLAVHSQGYGAHYQGVYPVKCNQDRFVVEDIVKFGSPFRFGLEAGSKPELLLAMSCLCKGSAE 196 Aridopsis ADC2 LQSAFDYAIKSQGYDSHYQGVYPVKCNQDRFVVEDIVKFGSSFRFGLEAGSKPEILLAMSCLCKGSPD 19 Grden pe ADC IHAAFDGAIQLQGYESHYQGVYPVKCNQDRYIVEDIVEFGSSFRFGLEAGSKPELLLAMSCLCKGNRE 24 Aridopsis ADC1 LQSAFDYAIQSQGYDSHYQGVYPVKCNQDRFIIEDIVEFGSGFRFGLEAGSKPEILLAMSCLCKGNPE 179 CADC1 ALLVCNGFKDTEYISLALIARKLLLNTVIVLEQEEELDLVIDISRKMAVRPVIGLRAKLRTKHSGHFG 265 Tocco ADC GLLVCNGFKDAEYISLALVARKLMLNTVIVLEQEEELDLVIDISHKMAVRPVIGLRAKLRTKHSGHFG 264 Aridopsis ADC2 AFLVCNGFKDAEYISLALLGRKLALNTVIVLEQEEELDLVIELSQKMNVRPVIGLRAKLRTKHSGHFG 258 Grden pe ADC AFLVCNGFKDSEYISLALIARKLALNTVIVLEQEEELDMVVEISNKLCIRPVIGVRAKLKTKHSGHFG 272 Aridopsis ADC1 AFLVCNGFKDSEYISLALFGRKLELNTVIVLEQEEELDLVIDLSQKMNVRPVIGLRAKLRTKHSGHFG 247 CADC1 STSGEKGKFGLTTTQILRVVKKLEGSGMLDCLQLLHFHIGSQIPTTELLADGVGEATQIYSELVRLGA 333 Tocco ADC STSGEKGKFGLTTTQIVRVVKKLEESGMLDCLQLLHFHIGSQIPSTGLLADGVGEAAQIYCELVRLGA 332 Aridopsis ADC2 STSGEKGKFGLTTTQIVRVVRKLRQSGMLDCLQLLHFHIGSQIPSTSLLSDGVAEAAQLYCELVRLGA 326 Grden pe ADC ATSGDKGKFGLTTIQILHVVKKLEQLDMLDCLQLLHFHIGSQIPTTELLADGVREASQIYCELLRLGA 34 Aridopsis ADC1 STSGEKGKFGLTTVQILRVVRKLSQVGMLDCLQLLHFHIGSQIPSTALLSDGVAEAAQLYCELVRLGA 315 CADC1 GMKFIDIGGGLGIDYDGSKSSDSDVSVAHGIEEYASAVVQAVQFVCDRKGVKHPVICSESGRAIVSHH 41 Tocco ADC GMKFIDIGGGLGIDYDGTKSCDSDVSVGYGIQEYASAVVQAVQYVGDRKGVKHPVICSESGRAIVSHH 4 Aridopsis ADC2 HMKVIDIGGGLGIDYDGSKSGESDLSVAYSLEEYAEAVVASVRVVCDRSSVKHPVICSESGRAIVSHH 394 Grden pe ADC QMKVLDIGGGLGIDYDGSKSGDSDESVAYGLEEYAAAVVHAVKYVCDRKNVKHPVICSESGRAIVSHH 48 Aridopsis ADC1 HMKVIDIGGGLGIDYDGSKSGESDLSVAYSLEEYAAAVVASVRFVCDQKSVKHPVICSESGRAIVSHH 383 CADC1 SIMIFEAVSASTTNVSPQLSSGGLQSLAETLNEDARADYRNLSAAAVRGEYDTCLLYSDQLKQRCVE 468 Tocco ADC SILIFEAVSASSTHVSSSHLSSGGLQSMAETLNEDALADYRNLSAAAVRGEYETCVLYSDQLKQRCVE 468 Aridopsis ADC2 SVLIFEAVSADKPMVHQATPGDIQFLLEGNEEARANYEDLYAAVMRGDHESCLLYVDQLKQRCVE 459 Grden pe ADC SILIFEASGASTNTAPSLSSIELQYLGEGLSEEALADYQNISAATLRGEYEACLLYTEQFKKRCVE 474 Aridopsis ADC1 SVLIFEAVSAGQQHETPTDHQFMLEGYSEEVRGDYENLYGAAMRGDRESCLLYVDQLKQRCVE 446 CADC1 QFNDGSLDIEQLAAVDSICDLVSKAIGVSDPVRAYHVNLSVFTSIPDFWAFGQLFPIVPIHRLDEKPT 536 Tocco ADC QFKEGSLGIEHLAAVDSICDFVSKAMGAADPVRTYHVNLSIFTSIPDFWAFGQLFPIVPIHRLDEKPA 536 Aridopsis ADC2 GFKEGVLSIEQLASVDGLCEWVLKAIGASDPVHTYNINLSVFTSIPDLWGIDQLFPIVPIHKLDQRPG 527 Grden pe ADC EFKQGTLGIEQLAAVDGLCDLITETIGVKDPVKKYHVNLSVFTSVPDFWGINQLFPIVPIHRLDEKPT 542 Aridopsis ADC1 GFKEGSLGIEQLAGVDGLCEWVIKAIGASDPVLTYHVNLSVFTSIPDFWGIDQLFPIVPIHKLDQRPA 514 CADC1 MRGILSDLTCDSDGKVDKFIGGESSLPLHDLGSNGGDGGRYYLGMFLGGAYEEALGGLHNLFGGPSVV 64 Tocco ADC VRGILSDLTCDSDGKVDKFIGGESSLPLHELGSNGDGGGYYLGMFLGGAYEEALGGLHNLFGGPSVV 63 Aridopsis ADC2 ARGILSDLTCDSDGKINKFIGGESSLPLHELDKNGSGGRYFLGMFLGGAYEEALGGVHNLFGGPSVV 594 Grden pe ADC ARGILSDLTCDSDGKIDKFIGGESSLPLHEMEGHGGGYYLGMFLGGSYEEALGGLHNLFGGPSVV 67 Aridopsis ADC1 ARGILSDLTCDSDGKINKFIGGESSLPLHEMDNNGCSGGRYYLGMFLGGAYEEALGGVHNLFGGPSVV 582 CADC1 RVLQSDSPHSFAVTRSVPGPSCADVLRAMQFEPELMFETLKHRAEESFDQEEEIEDKGLAFE 666 Tocco ADC RVVQSDSAHSFAMTRSVPGPSCADVLRAMQHEPELMFETLKHRAEEFLEQEDDKGLAVE 662 Aridopsis ADC2 RVSQSDGPHSFAVTRAVPGQSSADVLRAMQHEPELMFQTLKHRAEEMMNNNNKACGDKGNDKL 657 Grden pe ADC RVLQSDGPHGFAVTRAVAGSSCADVLRVMQHEPQLMFETLKHRALEFCGQHDDDSVVNAG 667 Aridopsis ADC1 RVLQSDGPHGFAVTRAVMGQSSADVLRAMQHEPELMFQTLKHRAEEPRHTKGGSEGENEEEEEDDEFN 65 CADC1 SLISGLAQSFHNMPYLVAPSSCCFTAATAKGGGYYYYNEDNAADCATAEDEIWSYCTA 724 Tocco ADC SLASSVAQSFHNMPYLVAPSSCRFTAATDNNGGYNYYYSDENAADSATGEDEIWSYCTA 721 (86%) Aridopsis ADC2 VVASCLAKSFNNMPYLSMETSTNALTAAVNNLGVYYCDEDVYDYISA 711 (71%) Grden pe ADC VLANSLAQSFDNMPYLVSSTTCCLNALTNNNGFYYCSGDDFSADTVSVATSVAGEDENWSY 728 (69%) Aridopsis ADC1 NVAASLDRSFHNMPYLATEQASPSNSLSAAISNLGFYYCDEAAAGGGGKGKDENWSYFG 72 (68%) Supplementl Figure S2.

A Lef Stem Root Flower Green fruit Red fruit B Slicylic cid 1 5 1 15 2 25h CADC1 CADC1 rrna rrna C Methyl jsmonte D Ethylene 1 5 1 15 2 25 h 1 5 1 15 2 25h CADC1 CADC1 rrna rrna Supplementl Figure S3.

A 35S:EV K154A (OD6=.2) (OD6=.2) (OD6=.2) B 35S:EV (OD6=.2) (OD6=.2) K154A (OD6=.2) Cell deth 35S:vrBsT (OD6=.5) / 35S:vrBsT (OD6=.5) / K154A / 35S:vrBsT 35S:vrBsT 35S:vrBsT 35S:vrBsT (OD6=.5) (OD6=.5) (OD6=.5) (OD6=.2) K154A / 35S:vrBsT (OD6=.5) 35S:vrBsT (OD6=.2) 35S:BAX (OD6=.5) / 35S:BAX (OD6=.5) 25 K154A / 35S:BAX (OD6=.5) Cell deth () () () () / K154A / 35S:BAX 35S:BAX 35S:BAX 35S:BAX (OD6=.5) (OD6=.5) (OD6=.5) (OD6=.2) Conductivity (μs cm1 ) 35S:BAX (OD6=.2) 2 d 15 c 1 d d 5 c Cell deth () () () Supplementl Figure S4. () 24 Hours fter infiltrtion 48 d

A IP: HA IP: cmyc AvrBsT:cMyc AvrBsT C222A:cMyc CADC1:HA AvrBsT:cMyc / AvrBsT C222A:cMyc αcmyc αha CADC1:HA CBB B 35S:EV 35S:vrBsT K154A (OD6=.2) (OD6=.2) (OD6=.2) (OD6=.2) C 35S:EV (OD6=.2) (OD6=.2) K154A (OD6=.2) 35S:vrBsT (OD6=.2) 35S:vrBsT C222A (OD6=.5) / 35S:vrBsT C222A (OD6=.5) K154A / 35S:vrBsT C222A (OD6=.5) () / K154A / 35S:vrBsT 35S:vrBsT 35S:vrBsT 35S:vrBsT C222A C222A C222A C222A (OD6=.5) (OD6=.5) (OD6=.5) (OD6=.2) Conductivity (μs cm1 ) 35S:vrBsT C222A (OD6=.2) Cell deth 25 15 1 5 Cell deth Supplementl Figure S5. 2 24 Hours fter infiltrtion 48

6 35S:EV (OD 6 =.2) (OD 6 =.2) 35S:vrBsT (OD 6 =.2) Amino cid (μmol g 1 FW) 5 4 3 2 1 c c c c c c c 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 di Asp Glu Asn Ser Gln Gly His Arg Thr Al GABA Pro Tyr Vl Ile Leu Phe Trp Lys Supplementl Figure S6.

Visile UV Visile UV cfu ml 1 TRV: TRV:CADC1 1 7 1 8 Xcv Ds1 Xcv Ds1 (EV) (vrbst) Xcv Ds1 Xcv Ds1 (EV) (vrbst) Xcv Ds1 Xcv Ds1 (EV) (vrbst) Xcv Ds1 Xcv Ds1 (EV) (vrbst) Supplementl Figure S7.

TRV: TRV:CADC1 3 Xcv Ds1 (EV) 2 Amino cid (μmol g 1 FW) 1 3 Xcv Ds1 (vrbst) 2 1 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 di Asp Glu Asn Ser Gln Gly His Arg Thr Al GABA Pro Tyr Vl Ile Leu Phe Lys Supplementl Figure S8.

8 Mock 1mM Alnine 1mM Alnine 1mM GABA 1mM GABA Xcv Ds1 (EV) Xcv Ds1 (vrbst) 6 Log cfu cm 2 4 2 8 16 24 8 16 24 Hours fter incution Supplementl Figure S9.

Arginine CADC1 AvrBsT Agmtine Pyrroline Putrescine GABA Spermidine H 2 O 2 Spermine NO Cell deth & defense response SA Supplementl Figure S1.