(Aloe vera L.) Downloaded from jcb.sanru.ac.ir at 20: on Thursday October 24th Liliaceae

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1 /7 / / (Aloe vera L.) (Aloe vera L.).... MS (2 ). P (1 ) I (0/25 ) /12/21 : 89/6/22 : IAA (0/1 ) (0/5 0/2 ) Kin (1-0/5 ). I (1 ) (1-0/2 ). 10/66 1/36 (2 ) + Kin (0/5 ) + (2 ). (4/81/16) (0/2 ) MS :..(3) (Aloe vera L.). Liliaceae

2 /5. I (6) 0/3 0/2. 2 (3) 2 0/5 10/1 0/ /8 0/53 0/5 (2). 0/2 0/5 2 Kin 15/39 0/2 +. 6/71 (4) 2. ) - ( ) ( ( ).(2) (5).(4) (1). MS

3 Kin I P D E SAS P< A B MS ph Kin MS C

4 74... A B C E D F -1 :B :A :D :C :F :E.

5 F s ** b a a c d c cd (2mgl-1)+Kin(0/5) (2mgl-1)+Kin(0/5)+(0/2mgl-1) (2mgl-1)+(0/5mgl-1) Kin (1mgl-1)+IAA(0/1mgl-1) P (1mgl-1)+I(0/25mgl-1) Kin (1mgl-1)+P(1mgl-1) P (1mgl-1)+(0/5mgl-1) Aloepolyphylla Aloe vera

6 /5 1...(F -1 ).(6 3 2) ) ( ) (.. 4/8 1/16.(E -1 4 ) 1 0/2. (6) 5/2 (0/5 ). 0/2 0/5. (3) IAA I 3. (2) 6/71 (0/5 ). 0/5 0/2

7 /7 / / -3 F s ** 7/09 6 / 1 12 / / / a 4 /8 1/ 16 3 /6 0/ 86 ab b 2 /8 0/ 75 ( 0/ 2mgl-1) 1 (1mgl-1) 2 I (1mgl-1) Abrie, A. and J.V. Staden Micropropagation of endangered Aloepolyphylla, Plant Growth Regulation, 33(1): Ahmed, S. and A.H. Kabir, M.B. Ahmed, M.A. Razvy and S. Ganesan Development of rapid micropropagation method of Aloe vera.lizvorniznanstveni rad. 24(2): Baksha, R. and M.A.A. Jahan, R. Khatun and J.L. Munshi Micropropagation of Aloe barbadensis Mill. hroughin vitro culture of shoot tip explants.plant issue Cult and Biotech. 15(2): Chaudhuri, S. and U. Mukundan Aloe Vera L. Micro propagation and Characterization of its gel. Phytomorphology, 51(2): Natali, L., I.C. Sanchez and A. Cavallini In vitro culture of Aloe barbadensismill.:micropropagation from vegetative meristems. Plant Cell, issue and Organ Culture 20(1): Liao, Z., M. Chen, F. an, X. Sun and K. ang Micro propagation of endangered Chinese aloe. Plant Cell, issue and Organ Culture 76(1):

8 78... In Vitro Micro Propagation of Aloe vera L. S. Golchoubian 1, Gh. Ranjbar 2 and S.K. Kazemitabar 2 1- Former M.Sc. Student, Sari Agricultural Sciences and Natural Resources University 2- Associate Professor, Sari Agricultural Sciences and Natural Resources University Abstract Aloe vera L. plant have industrial applications in various fields of health. Leaves of Aloe vera are used for treating against infectious diseases, wounds, burns, skin allergies and are also used to prevent human hair loss. Propagation of this plant has traditionally done by planting its shoot tips. Since Aloe vera plant is male sterile, it cannot produce fertile seeds for cultivation. Outcrosses will change its genetic uniformity, in which supposed as a very important character in production of this genotype. oday, micro propagation method is extensively used for reproduction of Aloe vera. o provide optimum in vitro propagation method, adventitious shoot explants of this plant were cultured on agar solid MS basal medium supplemented with sucrose. reatments used in the shooting stage include (2 ), Kin (0.5-1 ), ( ), IAA (0.1 ), I (0.25 ), P (1 ). Experiment was investigated sing a complete lyrandomized design. Results in shooting stage showed that the combination of (2 )+ Kin (0.5 )+ (2 ) with mean of in shooting stage and (2 ) by mean of in rooting stage produced the highest outcomes, respectively. Keywords: Aloe vera, Adventitious, MS medium, Micro propagation, Hormonal treatment