* 2 0 * 4 0 * 6 0 * 8 0 * * * * * * * * * 2 6 0

Transkript

1 Supplemental Figure 1. Protein alignment of the six members of the NPR family. Protein alignment was generated using MUSCLE software. Amino acid residues highlighted in back share similar chemical properties in all six members and residues highlighted in grey are common for some of the members. Numbers refer to the amino acid position on. BTB/POZ and ankyrin repeats domains are represented by dashed line box and solid line box, respectively. Conserved cysteines are indicated by solid black arrows. The five basic amino acids facilitating the nuclear localization of NPPR1 are indicated by empty black arrows. N P R 1 : N P R : N P R : N P R : B O P : B O P 1 : N P R 1 : N P R : N P R : N P R : B O P : B O P 1 : N P R 1 : N P R : N P R : N P R : B O P : B O P 1 : N P R 1 : N P R : N P R : N P R : B O P : B O P 1 : N P R 1 : N P R : N P R : N P R : B O P : B O P 1 : * 0 * 0 * 0 * 8 0 * * 1 0 * M D T T I D G F A D S Y E I S S T S F V A T D N T D S S I V Y L A A E Q V L T G P D V S A L Q L L S N S F E S V F D S P D D F Y S D A K L V L - S D G R E V S F H R C V L S A R S S F F K S A L A A A K K E K D S N N T A A V K L E L K M A T T T T T T T A R F S D S Y E F S N T S G N S F F A A E S S L D Y P T E - - F L T P P E V S A L K L L S N C L E S V F D S P E T F Y S D A K L V L - A G G R E V S F H R C I L S A R I P V F K S A L A T V K E Q K S S T T V K L Q L K M A T L T E P S S S L S F T S S H F - - S Y G S I G S N H F S S S - - S A S N P E V V S L T K L S S N L E Q L L S N S D C D Y S D A E I I V - - D G V P V G V H R C I L A A R S K F F Q D L F K K E K K I S K T E K P K Y Q L R M A A T A I E P S S S I S F T S S H L - - S N P S P V V T T Y H S A - - A N L E E L S S N L E Q L L T N P D C D Y T D A E I I I E E E A N P V S V H R C V L A A R S K F F L D L F K K D K D S S E - K K P K Y Q M K M S N L E E S L R S L S L D F L N L L I N G Q A - F S D V T F S V - - E G R L V H A H R C I L A A R S L F F R K F F C G T D S P Q P V T G I D P T Q H G S V P A S P T R G S T A P A M S N T F E E S L K S M S L D Y L N L L I N G Q A - F S D V T F S V - - E G R L V H A H R C I L A A R S L F F R K F F C E S D P S Q P G A E P A N Q T G S G A R A A A V G t L S 5 D g V H R C L A R s f F 1 0 * 1 0 * * 0 0 * 0 * 0 * 0 E I A K D Y E V G F D S V V T V L A Y V Y S S R V R P P P K G V S E C A D E N C C H V A C R P A V D F M L E V L Y L A F I F K I P E L I T L Y Q R H L L D V V D K V V I E D T L V I L K L A N I C G K A C M K L L D R C K E I I V K S N V D M V S L E K S L P E E E I A R D Y E V G F D S V V A V L A Y V Y S G R V R S P P K G A S A C V D D D C C H V A C R S K V D F M V E V L Y L S F V F Q I Q E L V T L Y E R Q F L E I V D K V V V E D I L V I F K L D T L C G T T Y K K L L D R C I E I I V K S D I E L V S L E K S L P Q H E M L P Y G A V A H E A F L Y F L S Y I Y T G R L K P F P L E V S T C V D P V C S H D C C R P A I D F V V Q L M Y A S S V L Q V P E L V S S F Q R R L C N F V E K T L V E N V L P I L M V A F N C K L T - - Q L L D Q C I E R V A R S D L Y R F C I E K E V P P E D L L P Y G N V G R E A F L H F L S Y I Y T G R L K P F P I E V S T C V D S V C A H D S C K P A I D F A V E L M Y A S F V F Q I P D L V S S F Q R K L R N Y V E K S L V E N V L P I L L V A F H C D L T - - Q L L D Q C I E R V A R S D L D R F C I E K E L P L E G I I P V N S V G Y E V F L L L L Q F L Y S G Q V S I V P Q K H E P R P N C G E R G C W H T H C S A A V D L A L D T L A A S R Y F G V E Q L A L L T Q K Q L A S M V E K A S I E D V M K V L I A S R K Q D M H - - Q L W T T C S H L V A K S G L P P E I L A K H L P I D G V I P V N S V G Y E V F L L L L Q F L Y S G Q V S I V P H K H E P R S N C G D R G C W H T H C T A A V D L S L D I L A A A R Y F G V E Q L A L L T Q K H L T S M V E K A S I E D V M K V L I A S R K Q D M H - - Q L W T T C S Y L I A K S G L P Q E I L A K H L P I E V g L 5 Y g P s C d C H C a D f L l V K E 1 l L C S K P * 8 0 * 0 0 * 0 * 0 * 0 * 8 0 * L V K E I I D R R K E L G L E V P K V K K H V S N V H K A L D S D D I E L V K L L L K E D H T N L D D A C A L H F A V A Y C N V K T A T D L L K L D L A D V N H R - N P R G Y T V L H V A A M R K E P Q L I L S L L E K G A S A S E A T I F K Q I I D I R E A L C L E P P K L E R H V K N I Y K A L D S D D V E L V K M L L L E G H T N L D E A Y A L H F A I A H C A V K T A Y D L L E L E L A D V N L R - N P R G Y T V L H V A A M R K E P K L I I S L L M K G A N I L D T T V A E K I K Q L R L I S P Q D E E T S P K I S E K L L E R I G K I L K A L D S D D V E L V K L L L T E S D I T L D Q A N G L H Y S V V Y S D P K V V A E I L A L D M G D V N Y R - N S R G Y T V L H F A A M R R E P S I I I S L I D K G A N A S E F T V L E K I K Q L R V K S V N I P E V E D K S I E R T G K V L K A L D S D D V E L V K L L L T E S D I T L D Q A N G L H Y A V A Y S D P K V V T Q V L D L D M A D V N F R - N S R G Y T V L H I A A M R R E P T I I I P L I Q K G A N A S D F T V V T K I E E L R L K S S I A R R S L M P H N H H H D L S V A Q D L E D Q K I R R M R R A L D S S D V E L V K L M V M G E G L N L D E S L A L H Y A V E S C S R E V V K A L L E L G A A D V N Y P A G P A G K T P L H I A A E M V S P D M V A V L L D H H A D P N V R T L V A K I E E L R L K S S M P L R S L M P - - H H H D L T S T L D L E D Q K I R R M R R A L D S S D V E L V K L M V M G E G L N L D E S L A L I Y A V E N C S R E V V K A L L E L G A A D V N Y P A G P T G K T A L H I A A E M V S P D M V A V L L D H H A D P N V Q T I R A L D S D E L V K L D L h 5 a L L a D V N G T L H A A P L A T 0 0 * 0 * 0 * 0 * 8 0 * * 5 0 L E G R T A L M I A K Q A T M A V E C N N I P E Q C K H S L K G R L C V E I L E Q E D K R E Q I - P R D V P P S F A V A A D E L K M T L L D L E N R V A L A Q R L F P T E A Q A A M E I A E M K G T C E F I V - T S L E P D R L T G T K R T S P G V K I A P F R I L E E L D G R T A L V I V K R L T K A D D Y K T S T E D G T P S L K G G L C I E V L E H E Q K L E Y L S P I E A S L S L P V T P E E L R M R L L Y Y E N R V A L A R L L F P V E T E T V Q G I A K L E E T C E F T A - S S L E P D H H I G E K R T S L D L N M A P F Q I H E K S D G R S A V N I L R R L T N P K D Y H T K T A K G R E S S K A R L C I D I L E R E I R K N P M - V L D T P M C S I S M P E D L Q M R L L Y L E K R V G L A Q L F F P T E A K V A M D I G N V E G T S E F T G - L S P P S S G L T G - N L S Q V D L N E T P H M Q T Q R F D G R S A V N I C R R L T R P K D Y H T K T S R K E P S - K Y R L C I D I L E R E I R R N P L V S G D T P T C S H S M P E D L Q M R L L Y L E K R V G L A Q L F F P A E A N V A M D V A N V E G T S E C T G L L T P P P S N D T T E N L G K V D L N E T P Y V Q T K R V G G I T P L D I L R T L T S D F L F K G A V P G L T H I E P N K L R L C L E L V Q S A A M V I S R E E G N N S N N Q N N D N N T G V D G I T P L D I L R T L T S D F L F K G A I P G L T H I E P N K L R L C L E L V Q S A A L V I S R E E G N N N S N D N N T M G I l T p p L L a e g t * 5 0 * 5 0 * * 0 0 * 0 * 0 * H Q S R L K A L S K T V E L G K R F F P R C S A V L D Q I M N C E D L T Q L A C G E D D T A E K R L Q K K Q R Y M E I Q E T L K K A F S E D N L - E L G N S S L T D S T S S T S K S T G G K R S N R K L S H R R R H L S R L R A L C K T V E L G K R Y F K R C S - - L D H F M D T E D L N H L A S V E E D T P E K R L Q K K Q R Y M E L Q E T L M K T F S E D K E - E C G K S S T P K P T S A V R S N R K L S H R R L K V D K R D F L K R P Y G N G D L L T R M V A L M K T V E T G R R F F P Y G S E V L D K Y M A E Y I D D D I L D D F H F E K G S T H E R R L K R M R Y R E L K D D V Q K A Y S K D K E S K I A R S C L S A S S S P S S S S I R D D L H N T T M L T R M K A L M K T V E T G R R Y F P S C Y E V L D K Y M D Q Y M D E E I P D M S Y P E K G T V K E R R Q K R M R Y N E L K N D V K K A Y S K D K V A R S C L S S S S P A S S L R E A L E N P T I Y P H M N E E H N S G S S G G S N N N L D S R L V Y L N L G A G T G Q M G P G R D Q G D D H - - N S Q R E G M S R H H H H H Q D P S T M Y H H H H Q H H F I Y P R M K D E H T S G S S L D S R L V Y L N L G A T N R D I G D D N - - S N Q R E G M N - L H H H H H D P S T M Y H H H H H H F p Y D : 5 9 : 0 0 : 5 8 : 5 7 : 9 1 : 7 : : 1 1 : 1 0 : : 8 7 : 8 : : : : 8 : 1 7 : 1 : 5 9 : 5 8 : 5 5 : : 9 : : 8 9 : 8 9 : 8 : 7 7 : 1 5 : 0 5

2 Supplemental Figure. Schematic representation of cis-acting element in the promoters of NPR family genes. The blue lines represent the promoter regions of the six members (about 1.5kb upstream of start codon). Putative TATA boxes (yellow blocks), CAAT boxes (blue blocks) and W boxes (red blocks) were identified by querying the PLACE database. ( NPR NPR NPR BOP BOP1 100 bp TATA box CAAT box W box

3 Supplemental Figure. Expression profiles of NPR family members from microarray database resource Genevestigator. Summary of relative expression levels (Log ) of NPR family genes in various tissues of Arabidopsis, generated by Genevestigator software. The expression of each gene is represented by its designated color. Arabidopsis thaliana callus cell culture / primary cel l sperm cel l seedlin g cotyledons hypocotyl radicle imbibed seed inflorescence flower carpel ovar y stigma petal sepal stamen pollen abscission zone pedicel silique seed embryo endosper m micropylar endosperm peripheral endosper m chalazal endosper m testa (seed coat ) general seed coat chalazal seed coat suspensor stem node shoot apex cauline leaf rosette juvenile leaf adult leaf petiole senescent leaf hypocotyl xylem cork leaf primordi a stem roots lateral root root hair zone root tip elongation zone endodermi s endodermis+corte x epid. atrichoblast s lateral root cap stel e NPR NPR NPR BOP BOP1 arrays Log Relative Expression Levels

4 Supplemental Figure. Bacterial populations (genotypes indicated on x-axis) day 0 after inoculation with virulent P.s.t. DC000. Data represent mean ± SE of three replicates, each consisting of four infected inflorescences. A, Flowers of six-week-old Col-0, npr1-, npr-, npr- and npr- npr- (as in Fig. H). B, Flowers of six-week-old Col-0, npr1-, npr- and npr1- npr- double mutant (as in Fig. K). C, Leaves of four-week-old Col-0 and five independent transgenic lines overexpressing NPR (as in Fig. 5C). D, Flowers of an F (Col-0 X npr-) segregating population of 9 plants, including Col-0, 5 heterozygous NPR/npr-, and 7 homozygous npr mutants (as in Fig. 7E). A 5 B 5 Log 10 Pseudomonas syringae (cfu mg FW -1 ) 1 Log 10 Pseudomonas syringae (cfu mg FW -1 ) 1 0 Col-0 npr1- npr- npr- npr- npr- 0 Col-0 npr1- npr- npr1- npr- C 5 D 5 Log 10 Pseudomonas syringae (cfu mg FW -1 ) 1 Log 10 Pseudomonas syringae (cfu mg FW -1 ) 1 0 Col-0 5S:NPR T1-1 5S:NPR T1-5S:NPR T1-5S:NPR T1-5S:NPR T1-5 0 Col-0 NPR/npr- npr-

5 Supplemental Figure 5. Additional negative control for transient BiFC analysis (Fig. ). A, Representative bright field image. B through F, Representative confocal images of onion epidermal cells co-bombarded with constructs expressing B, YFPC and NPR:YFPN, C,YFPN and TGA:YFPC, D, YFPC and :CFPN, E, CFPN and TGA:YFPC, F, YFPN and :YFPC. All of them served as extra negative control for transient BiFC assay in Fig. to rule out that each YFP half alone can interact with NPR, TGA or. A C E B D F

6 Supplemental Dataset 1. Protein sequences used to generate the phylogenetic tree presented in Figure 1B. >AT1G80 () MDTTIDGFADSYEISSTSFVATDNTDSSIVYLAAEQVLTGPDVSALQLLSNSFESVFDSPDDFYSD AKLVLSDGREVSFHRCVLSARSSFFKSALAAAKKEKDSNNTAAVKLELKEIAKDYEVGFDSVVT VLAYVYSSRVRPPPKGVSECADENCCHVACRPAVDFMLEVLYLAFIFKIPELITLYQRHLLDVVD KVVIEDTLVILKLANICGKACMKLLDRCKEIIVKSNVDMVSLEKSLPEELVKEIIDRRKELGLEVP KVKKHVSNVHKALDSDDIELVKLLLKEDHTNLDDACALHFAVAYCNVKTATDLLKLDLADVN HRNPRGYTVLHVAAMRKEPQLILSLLEKGASASEATLEGRTALMIAKQATMAVECNNIPEQCKH SLKGRLCVEILEQEDKREQIPRDVPPSFAVAADELKMTLLDLENRVALAQRLFPTEAQAAMEIAE MKGTCEFIVTSLEPDRLTGTKRTSPGVKIAPFRILEEHQSRLKALSKTVELGKRFFPRCSAVLDQI MNCEDLTQLACGEDDTAEKRLQKKQRYMEIQETLKKAFSEDNLELGNSSLTDSTSSTSKSTGGK RSNRKLSHRRR >ATG10 (NPR) MATTTTTTTARFSDSYEFSNTSGNSFFAAESSLDYPTEFLTPPEVSALKLLSNCLESVFDSPETFYS DAKLVLAGGREVSFHRCILSARIPVFKSALATVKEQKSSTTVKLQLKEIARDYEVGFDSVVAVLA YVYSGRVRSPPKGASACVDDDCCHVACRSKVDFMVEVLYLSFVFQIQELVTLYERQFLEIVDKV VVEDILVIFKLDTLCGTTYKKLLDRCIEIIVKSDIELVSLEKSLPQHIFKQIIDIREALCLEPPKLERH VKNIYKALDSDDVELVKMLLLEGHTNLDEAYALHFAIAHCAVKTAYDLLELELADVNLRNPRG YTVLHVAAMRKEPKLIISLLMKGANILDTTLDGRTALVIVKRLTKADDYKTSTEDGTPSLKGGLC IEVLEHEQKLEYLSPIEASLSLPVTPEELRMRLLYYENRVALARLLFPVETETVQGIAKLEETCEFT ASSLEPDHHIGEKRTSLDLNMAPFQIHEKHLSRLRALCKTVELGKRYFKRCSLDHFMDTEDLNHL ASVEEDTPEKRLQKKQRYMELQETLMKTFSEDKEECGKSSTPKPTSAVRSNRKLSHRRLKVDKR DFLKRPYGNGD >AT5G5110 (NPR) MATLTEPSSSLSFTSSHFSYGSIGSNHFSSSSASNPEVVSLTKLSSNLEQLLSNSDCDYSDAEIIVDG VPVGVHRCILAARSKFFQDLFKKEKKISKTEKPKYQLREMLPYGAVAHEAFLYFLSYIYTGRLKP FPLEVSTCVDPVCSHDCCRPAIDFVVQLMYASSVLQVPELVSSFQRRLCNFVEKTLVENVLPILM VAFNCKLTQLLDQCIERVARSDLYRFCIEKEVPPEVAEKIKQLRLISPQDEETSPKISEKLLERIGKI LKALDSDDVELVKLLLTESDITLDQANGLHYSVVYSDPKVVAEILALDMGDVNYRNSRGYTVL HFAAMRREPSIIISLIDKGANASEFTSDGRSAVNILRRLTNPKDYHTKTAKGRESSKARLCIDILER EIRKNPMVLDTPMCSISMPEDLQMRLLYLEKRVGLAQLFFPTEAKVAMDIGNVEGTSEFTGLSPP SSGLTGNLSQVDLNETPHMQTQRLLTRMVALMKTVETGRRFFPYGSEVLDKYMAEYIDDDILD DFHFEKGSTHERRLKRMRYRELKDDVQKAYSKDKESKIARSCLSASSSPSSSSIRDDLHNTT >ATG190 (NPR) MAATAIEPSSSISFTSSHLSNPSPVVTTYHSAANLEELSSNLEQLLTNPDCDYTDAEIIIEEEANPVS VHRCVLAARSKFFLDLFKKDKDSSEKKPKYQMKDLLPYGNVGREAFLHFLSYIYTGRLKPFPIEV STCVDSVCAHDSCKPAIDFAVELMYASFVFQIPDLVSSFQRKLRNYVEKSLVENVLPILLVAFHC

7 DLTQLLDQCIERVARSDLDRFCIEKELPLEVLEKIKQLRVKSVNIPEVEDKSIERTGKVLKALDSD DVELVKLLLTESDITLDQANGLHYAVAYSDPKVVTQVLDLDMADVNFRNSRGYTVLHIAAMRR EPTIIIPLIQKGANASDFTFDGRSAVNICRRLTRPKDYHTKTSRKEPSKYRLCIDILEREIRRNPLVS GDTPTCSHSMPEDLQMRLLYLEKRVGLAQLFFPAEANVAMDVANVEGTSECTGLLTPPPSNDTT ENLGKVDLNETPYVQTKRMLTRMKALMKTVETGRRYFPSCYEVLDKYMDQYMDEEIPDMSYP EKGTVKERRQKRMRYNELKNDVKKAYSKDKVARSCLSSSSPASSLREALENPT >ATG170 (BOP) MSNLEESLRSLSLDFLNLLINGQAFSDVTFSVEGRLVHAHRCILAARSLFFRKFFCGTDSPQPVTGI DPTQHGSVPASPTRGSTAPAGIIPVNSVGYEVFLLLLQFLYSGQVSIVPQKHEPRPNCGERGCWHT HCSAAVDLALDTLAASRYFGVEQLALLTQKQLASMVEKASIEDVMKVLIASRKQDMHQLWTTC SHLVAKSGLPPEILAKHLPIDVVTKIEELRLKSSIARRSLMPHNHHHDLSVAQDLEDQKIRRMRRA LDSSDVELVKLMVMGEGLNLDESLALHYAVESCSREVVKALLELGAADVNYPAGPAGKTPLHI AAEMVSPDMVAVLLDHHADPNVRTVGGITPLDILRTLTSDFLFKGAVPGLTHIEPNKLRLCLELV QSAAMVISREEGNNSNNQNNDNNTGIYPHMNEEHNSGSSGGSNNNLDSRLVYLNLGAGTGQMG PGRDQGDDHNSQREGMSRHHHHHQDPSTMYHHHHQHHF >ATG5710 (BOP1) MSNTFEESLKSMSLDYLNLLINGQAFSDVTFSVEGRLVHAHRCILAARSLFFRKFFCESDPSQPGA EPANQTGSGARAAAVGGVIPVNSVGYEVFLLLLQFLYSGQVSIVPHKHEPRSNCGDRGCWHTHC TAAVDLSLDILAAARYFGVEQLALLTQKHLTSMVEKASIEDVMKVLIASRKQDMHQLWTTCSY LIAKSGLPQEILAKHLPIELVAKIEELRLKSSMPLRSLMPHHHDLTSTLDLEDQKIRRMRRALDSS DVELVKLMVMGEGLNLDESLALIYAVENCSREVVKALLELGAADVNYPAGPTGKTALHIAAEM VSPDMVAVLLDHHADPNVQTVDGITPLDILRTLTSDFLFKGAIPGLTHIEPNKLRLCLELVQSAAL VISREEGNNNSNDNNTMIYPRMKDEHTSGSSLDSRLVYLNLGATNRDIGDDNSNQREGMNLHH HHHDPSTMYHHHHHHF >OS01G09880 MSSNGKPNPPPAASAAAAAGNGAGGPPKMYQRPIYRPQAPAAKRRRGGRSSCRFSCCCCFFYAV LVVLLLAFVAAVAGGAFYLLYRPHRPAFTLSVARVDKLSLSSSATAPTLTDSIDVTLTAKNPNKK LVYLYDDFAVTAATAANAVPLGEGSVPGFVHDAGNITVIKATVSASALGVDPTTAATDIKKSGE FTITLDLETKAGVKVGGLKTKKIGVLVHCEGIKVAAPAPPPPPAKKKKGGVKLSVSDAPSPAASV DDTTPSPPPATTVARVCQVRIRVKIWKWTF >OS0G0 METSTISFSSSSPPSPPPPQPAPGDIDAVSLGRLSRNLENLLDPAFLNCADAEIVLASGGGDPGGGA VVGVHRCILAARSRFFYDHFSSAPAPAPATAGDKPQLDLDGLVPGGRHIGRDALVAVLSYLYTG RLRSAPPEAAACLDDGCSHDACRPAIDFVVESTYAASGFQISELVSLFQRRLSDFVNKALAEDILP ILVVASTCHLPELLNQCIQRVANSNLDNRYLEKRLPDDLYAKLKEFRVPDEPHSGILDPEHEKRV RNIHKALDSDDVDLVGMLLKESPVTLDDAFAIHYAAAYCEPKVLAELLKLESANVNLKNSSGYT PLHMACMRREPDIIVSLIEKGASVLERTQDGRDALTICKRLTREKDRNEKSEKCKERSKAYLCIG VLQQEIKRRPQILEDQMSAEESIATPLLVDNFHMRLLNLENRVAFARIFFPSEAKLVMRIAQADST QEFAGLTSANFSKLKEVDLNETPTMQNRRLRERLDALTKTVELGRRYFPHCSEVLDKFLNEESTD

8 LILLESGTAEDQQTKRMRFSELREDVRKAFTKDKAAGAAISSSTSASSSPRYETKLRPGNKKGKL SR >OS01G500 MPARSAVVVIAMEPSSSITIASSSSYLSNGSSPCSVSLAPPGAGAVAAQAAPVAAGEGGGGGGGG GGGGSSSVEVVSLNRLSANLERLLLDSDLDCSDADVDVADGGPPVPVHRCILAARSTFFYNLFA ARGRGGDGAAGGGGGGGGGGGERTGGRPRYKMEELVPGGRVGRDAFLSLLGYLYTGKLRPAP DDVVSCADPMCPHDSCPPAIRFNVEQMYAAWAFKITELISLFQRRLLNFVDKTLVEDVLPILQVA FHSELTPVLEKCIRRIARSNLDNVSLDKELPPEVAVQIKEIRQKSQPNEGDTVISDPVHEKRVRRIH RALDSDDVELVKLLLNESEITLDDANALHYAAAYCDSKVVSELLDLRLANLNLKNSRGYTALHL AAMRREPAIIMCLLNKGAAVSQLTADGQSAMSICRRLTRMKDYNTKMEQGQESNKDRLCIDILD REMIRKPMAVEDSVTSPLLADDLHMKLLYLENRVAFARLFFPAEAKVAMQIAQADTTPEFGIVP AASTSGKLKEVDLNETPVTQNKRLRSRVDALMKTVELGRRYFPNCSQVLDKFLEDDLPDSPDAL DLQNGTSDEQNVKRMRFCELKEDVRKAFSKDRADNSMFSILSSSSSSSPPPKVAKK >OS11G0800 MEAPGELLLLSMAAFATAHPGERASIQAALGSLPRAPSSTMYYRFGSHGLQVLGENREPSASGG GGQAQATQQQQGTSSGVADNRREHAFDFGVGSSSSSSGRRHAMGAAPSVISGSKRVAREE GGHSCGFEFEGSDQGRHAARGQACGLGGLDQGPSGGSAHDEDARGYGFGDARCVGQRKDAAP THQLRIGAGRDQGRSVRLRRDAREDGRGRRLEEGNRAGDGYVAASVQAHGDACVEDQGAGSS SVQQLPSSVDGSDGMIAFEVGKELTLHGSVVSSPWNELWLGKPLIFHDLDLVRAMKDQGGMPN ERIDLSGKITSILLSHPGPVSYFRIDSSVINNGAQQKIEEWCDVLRKKNVETVVMANCQWPSHPIE FPLQSLNCSSLRTLHLCFFNIPDMYLDHVSSLAVIDLACCRISDENLFALVCQCVSLRELDIGMFSE GKERIRSESLKFLQIWRSSVSHITIQWAPKLEKVIIGAAQGMKSFSSRTSSSTWISILGAPMLREVW FNLSSQTRSIDNVYLDVGHVPITSLRKLELSIAFKERKGRHALLNFFRSCTELKELVLWREDKVYF EEECDVHSDDWSSALKDIACLKSHLQVLKLFDYGGGETEIAIASAVLEHGASIENLTIMSTTSNAD DILSQAKQKLEKVERDSQITGDKKKYSIRDSQENWRQKIQIIQRVAVKQALIPREFIQSINSPRGLR FLLRGWGSSRVTDPAGAVAQDAELMESVGAGHRPDVVALLEILEAHRAALLCRLAHSQVVKIA AGECMFHGVRGGGGGGGGGILAKVAGLHTMHWRIPYHPTRDLLDEVALGICRGGCGIHACAN ASRSRG >OS1G010 MSSEDSLKSLSLDYLNLLINGQAFSDVAFSVEGRLVHAHRCVLAARSLFFRKLFCGLDPNHQPPP PPPPPLNWPTAGGGGGGSGGGGRGGAGGGGGAPATPELVIPVSSIRYEVLVLVLQFLYSGQASV AAPKSGPLPGCGARGCWHTRCGAAVDLALDTLAAARSFGVEQLALLVQKQLESMVKEASVDD VMKVLMASRKFEMQELWATCSHLVARSGLSADLLAKHLPIDVVAKIEEIRAKSPLAAAAAPRSP FLTHHYLPMNPASSAADRDNKIRRMRRALDAADIELVKLMVMGEGLDLDDALAVHYAVQHCN RDVVKALLELGAADVNSRAGPTGKTALHLAAEMVSPDMVSVLLDHHADPNSRTLDGVTPLDV LRSLTSEFLFKGAVPGLTHIEPNKLRLCLELVQSAVMVTTRDDGAPVTGGEAGGSDGGNFPRSD ADDSLVSLTMNSTLMYQGQEMAAAVAAGEGRKSNNGRGSPPPAMYFPNGFA > OS01G700

9 MEETLKSLSMDYLNLLINGQAFSDVTFSVEGRLVHAHRCILAARSLFFRKFFCGAAADQAAAPP GALLLDHLSPRSPSGGASASSPRGAGGSAAAAAAATPGAVIPVSSVSYEVFLLLLQFLYSGQVSL VPQKGEPRPGCGERGCWHTHCAAAVDLALDTLAAARSFGVEELALLTQKQLAGMVEKASIEDV MKVLMASRKQDLHQLWTTCSHLVAKSGLPPEVLAKHLPIDVVAKIDELRLKSMSRRSPFLSHHH HHPHAAAAGIEASSAAELDDHHKIRRMRRALDSSDVELVKLMVMGEGLNLDDALALHYAVEN CSREVVKALLELGAADVNHPAGPAGKTPLHVAAEMVCPDMVAVLLDHHADPNVRTVDGVTPL DILRTLTSDFLFKGAVPGLAHIEPNKLRLCLELVQSAAMVMSREDAQTAAVNAAPIYGESPGGG GGGGVYNASGTSSSMVNLSLDNRMVYLNLGMDAQFGKMNDGGDGDDGGSRGPSSLFSPHGFP >ATG750 (CAO) MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTS SVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWW TAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGL TALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVIN VLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAV AESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE

10 Supplemental Table 1. Primer sequences used in this study. Table 1. Primer sequences used for npr- and npr- genotyping. Primer Name Primer Sequences LP 5'-AGTGATCCGAAAGTTGTTGCC-' RP 5'-CCCTTCTCAAAGAAATCG-' LP 5'-TGTGTTCCATTTCTCCTG-' LBa1 5'-TGGTTCACGTAGTGGGCCATCG-' Supplemental Table. Primer sequences used to amplify promoters of NPR genes and cdna clones used. Gene Name Primer Sequences BAC Clone ID NPR NPR NPR BOP BOP1 5 -CGAAGCTTTTTGGCAATTGGGTGTTT- 5 -ATCCGAATCGGCGAATCCATCAA- 5 -GCAAGCTTAAGAAAGCAAGACCAAAA- 5 -CGCCGGCATCACGAGATCGGCG- 5 -GCAAGCTTTAGCCTTGTAGGTAGAGT- 5 -CTCCGCCTTCTTGTAGAATTATCA- 5 -CGAAGCTTCTTGGCTTGAGAGGTTTG- 5 -CGCCGTTTGTTCCCCAGAAGTAT- 5 -CGAAGCTTAACCCATAGTATTTCTTA- 5 -CGCCGAGTTGCCTCCTTTTATTG- 5 -GCAAGCTTTTCTGCGATAATGGT- 5 -GCCCGAGCTCCTTTGTTGATTTC- BAC F15H1 BAC F0B18 BAC K17O BAC T1H5 BAC F1H10 BAC FI1 Supplemental Table. Primer and probe sequences for TaqMan Assays of NPR genes in different plant tissues. Gene Name Primer Sequences Probe Sequences NPR NPR NPR BOP BOP1 Ubiquitin Actin 5'-GTCACGTGCTGTCTGACCTTGT-' 5'-TCACAGTTCATAATCTGGTCGAGC-' 5'-TTGCGTAGACGACGATTGTTG-' 5'-AAAGAACCTCCACCAAATCC-' 5'-AAGGTCCAAAAGGCATA-' 5'-AAGACAAGACCGCGCAATCT-' 5'-CACCCACTTGTTCCCATTCG-' 5'-CTGAGCAAGTCCCACTCGCT-' 5'-GAGCTGCCGTGAATTATCC-' 5'-CAGCCGCGTGCAA-' 5'-GGCCGTCTAGTCCACGCT C-' 5'-GAATTTGCGGAAGAAGAGGCT-' 5'-AGGCCTCAACTGGTTGCTGT-' 5'-ACCGGCAAGACCATCACTCT-' 5'-GATTCAGCCCAGAAGTCTTG-' 5'-TCTCGTGGATTCCAGCAGCT-' 5'-TYCCGCGCTGTTCGGCAGT-' 5'-CACGTGGCTTGCCGGTCAAAG G-' 5'-AGCAAAGACAAAGAGTCT-' 5'-TGCCCGAGGATCTCCAAAGGTT-' 5'-CGGGTCCGGCAGGGAAAACAC-' 5'-CCGTTGCATCCTCGCTGCTCG-' 5'-CGAGAGCAGCGACACCCATCGACA-' 5'-CCAGCCCTCGTTTGTGGGAAAGG-' Supplemental Table. Primer sequences used for amplifying cdna fragments for dotblot analysis.

11 Gene Name NPR PR1 Ubiquitin Primer Sequences 5 - CGTGTGCTCTTCATTTCGCTG- 5 - ATCGTTTCCCGAGTTCCACGGTTT- 5 - AGTGATCCGAAAGTTGTTGCCGAG- 5 - ACCTTCGACCAGTCTCAACTGT- 5 - CTCGAAAGCTCAAGATAGCCCACA- 5 - CTTCTCGTTCACATAATTCCCACG- 5 - ACCGGCAAGACCATCACTCT- 5 - AGGCCTCAACTGGTTGCTGT- Supplemental Table 5. Primer sequences used for BiFC vector construction and vector names. Gene Name Primer Sequences and cloning site BiFC Vector Name TGA NPR 5 -GCGAGCTCCCGACACCACCAT (SacI) 5'-GCCCGCGGCACCGACGACGAG-' (SacII) psata-ncerulean-n (pe7) 5 -GCGAGCTCCCGACACCACCAT-' (SacI) 5 -GCCCGCGGCACCGACGACGAG-'(SacII) psata-ceyfp-n1 (pe) 5 -GCGAATTCCGCTGATACCAGT-' (EcoRI) 5'-GTACCACTCTCTGGGTCGAGC-' (KpnI) 5'-GCGAGCTCCCGCTACTTTGAC-' (SacI) 5'-ATCCGCGGCTTGTGTTGTGC-' (SacII) psata-ceyfp-n1 (pe) psata-neyfp-n1 (pe085)