Supplementary information for. MATE-Seq: Microfluidic Antigen-TCR Engagement Sequencing

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Electronic Supplementary Material (ESI) for Lab on a Chip. This journal is The Royal Society of Chemistry 2019 Supplementary information for MATE-Seq: Microfluidic Antigen-TCR Engagement Sequencing Alphonsus H.C. Ng, 1,2, Songming Peng, 1, Alexander M. Xu, 1,2, Won Jun Noh, 3 Katherine Guo, 1 Michael T. Bethune, 3 William Chour, 2,3 Jongchan Choi, 2,4 Sung Yang, 4 David Baltimore, 3 and James R. Heath 2,* 1 Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Blvd, Pasadena, CA 91125, USA 2 Institute for Systems Biology, Seattle, WA 98109, USA. 3 Division of Biology and Biological Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA 4 School of Mechanical Engineering, Gwangju Institute of Science and Technology, 123 Cheomdangwagiro, Buk-gu, Gwangju, 61005, South Korea These authors contributed equally to this work. * Corresponding Author E-mail: jheath@systemsbiology.org

Supplementary Figure 1: Bright field and fluorescent micrographs of pnps. The pnps are labeled with three different DNA (PS1-PI-Cα, PS1-PI-Cβ, NP-DNA) at the same time. Complementary DNA for Cα (Red), Cβ (Green) and MHC-DNA (Yellow) are hybridized to the DNA on the pnp and give the correct fluorescent emission, indicating successful DNA labeling on the particles.

Supplementary Figure 2: Bright field and fluorescent micrographs of viability-stained NY-ESO-specific Jurkat cells pulldown by NY-ESO-specific pnps.

Supplementary Figure 3: A movie screen capture of DLD sorting of viability-stained cells using a device with circular (left) or I-shaped pillars (right). As a cell passes the dotted yellow line in the device, the event is recorded as a spot on the corresponding kymograph, below. The fraction of cells that go to the droplet generator is the sorting efficiency of DLD.

Supplementary Figure 4: Viability-stained donor PBMCs after processing by MATE-seq without lysis reagent. Droplets with single cells are indicated by red boxes in the bright field micrograph at the top, and their viability is verified by the fluorescence at the bottom. Scale bar is 40 µm.

Supplementary Table 1: DNA sequences used for NP labeling Name PS1-PI-Cα PS1-PI-Cβ NP-DNA DNA sequence /5PCBio/TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNXXXXXXTCTCT CAGCTGGTACACGGC /5PCBio/TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNXXXXXXGATGG CTCAAACACAGCGACCTC /5BiosG/AAAAAAAAACTATGTCGATACAAGTCAGATAGTTCAACTCGTTCACTATAACT GAATCCTCGGGATGCCTA N is either one of A, T, G or C XXXXXX is either one of ACTCTT, CGAGTC, TAGACG or TTCAGG, representing peptide identifiers for CMV, MHC-J, MART-1, or EBV, respectively. CTGAATCCTCGGGATGCCTA is the sequence that hybridizes to SAC-DNA Supplementary Table 2: Vα-gene-specific primers for cloning TCRα genes TRAV gene α ID Vα TRAV1-1*01 5'-TACAGGAAGCCTCAGCA GGACAAAGCCTTGAGCAGCCCTC-3' TRAV1-2*01 5'-TACAGGAAGCCTCAGCA GGACAAAACATTGACCAGCCCACTG-3' TRAV2*01 5'-TACAGGAAGCCTCAGCA AAGGACCAAGTGTTTCAGCCTTCCAC-3' TRAV3*01 5'-TACAGGAAGCCTCAGCA GCTCAGTCAGTGGCTCAGCCGGA-3' TRAV4*01 5'-TACAGGAAGCCTCAGCA CTTGCTAAGACCACCCAGCCCATC-3' TRAV5*01 5'-TACAGGAAGCCTCAGCA GGAGAGGATGTGGAGCAGAGTCTTTTCC-3' TRAV6*01 5'-TACAGGAAGCCTCAGCA AGCCAAAAGATAGAACAGAATTCCGAGGC-3' TRAV6*03 5'-TACAGGAAGCCTCAGCA GAGGCCCTGAACATTCAGGAGGG-3' TRAV7*01 5'-TACAGGAAGCCTCAGCA GAAAACCAGGTGGAGCACAGCCC-3' TRAV8-1*01 5'-TACAGGAAGCCTCAGCA GCCCAGTCTGTGAGCCAGCATAACC-3' TRAV8-2*01 5'-TACAGGAAGCCTCAGCA GCCCAGTCGGTGACCCAGCTTG-3' TRAV8-2*02 5'-TACAGGAAGCCTCAGCA GCCCAGTCGGTGACCCAGCTTAG-3' TRAV8-3*01 5'-TACAGGAAGCCTCAGCA GCCCAGTCAGTGACCCAGCCTG-3' TRAV8-4*06 5'-TACAGGAAGCCTCAGCA CTCTTCTGGTATGTGCAATACCCCAACC-3' TRAV8-4*07 5'-TACAGGAAGCCTCAGCA GTTGAACCATATCTCTTCTGGTATGTGCAATACC-3' TRAV8-6*01 5'-TACAGGAAGCCTCAGCA GCCCAGTCTGTGACCCAGCTTGAC-3' TRAV8-7*01 5'-TACAGGAAGCCTCAGCA ACCCAGTCGGTGACCCAGCTTG-3' TRAV9-1*01 5'-TACAGGAAGCCTCAGCA GGAGATTCAGTGGTCCAGACAGAAGGC-3' TRAV9-2*01 5'-TACAGGAAGCCTCAGCA GGAAATTCAGTGACCCAGATGGAAGG-3' TRAV9-2*02 5'-TACAGGAAGCCTCAGCA GGAGATTCAGTGACCCAGATGGAAGG-3' TRAV10*01 5'-TACAGGAAGCCTCAGCA AAAAACCAAGTGGAGCAGAGTCCTCAGTC-3' TRAV11*01 5'-TACAGGAAGCCTCAGCA CTACATACACTGGAGCAGAGTCCTTCATTCC-3' TRAV12-1*01 5'-TACAGGAAGCCTCAGCA CGGAAGGAGGTGGAGCAGGATCC-3' TRAV12-2*01 5'-TACAGGAAGCCTCAGCA CAGAAGGAGGTGGAGCAGAATTCTGG-3' TRAV12-2*03 5'-TACAGGAAGCCTCAGCA GGACCCCTCAGTGTTCCAGAGGG-3' TRAV12-3*01 5'-TACAGGAAGCCTCAGCA CAGAAGGAGGTGGAGCAGGATCCTG-3' TRAV13-1*02 5'-TACAGGAAGCCTCAGCA GGAGAGAATGTGGAGCAGCATCCTTC-3' TRAV13-2*01 5'-TACAGGAAGCCTCAGCA GGAGAGAGTGTGGGGCTGCATCTTC-3' TRAV14/DV4*01 5'-TACAGGAAGCCTCAGCA GCCCAGAAGATAACTCAAACCCAACCAG-3' TRAV14/DV4*04 5'-TACAGGAAGCCTCAGCA CAGAAGATAACTCAAACCCAACCAGGAATG-3' TRAV16*01 5'-TACAGGAAGCCTCAGCA GCCCAGAGAGTGACTCAGCCCGA-3' TRAV17*01 5'-TACAGGAAGCCTCAGCA AGTCAACAGGGAGAAGAGGATCCTCAGG-3' TRAV18*01 5'-TACAGGAAGCCTCAGCA GGAGACTCGGTTACCCAGACAGAAGG-3' TRAV19*01 5'-TACAGGAAGCCTCAGCA GCTCAGAAGGTAACTCAAGCGCAGACTG-3' TRAV20*01 5'-TACAGGAAGCCTCAGCA GAAGACCAGGTGACGCAGAGTCCC-3' TRAV21*01 5'-TACAGGAAGCCTCAGCA AAACAGGAGGTGACGCAGATTCCTGC-3' TRAV22*01 5'-TACAGGAAGCCTCAGCA GGAATACAAGTGGAGCAGAGTCCTCCAG-3' TRAV23/DV6*01 5'-TACAGGAAGCCTCAGCA CAGCAGCAGGTGAAACAAAGTCCTCA-3' TRAV23/DV6*04 5'-TACAGGAAGCCTCAGCA CAGCAGGTGAAACAAAGTCCTCAATCTTTG-3'

TRAV24*01 5'-TACAGGAAGCCTCAGCA ATACTGAACGTGGAACAAAGTCCTCAGTCAC-3' TRAV25*01 5'-TACAGGAAGCCTCAGCA GGACAACAGGTAATGCAAATTCCTCAGTACC-3' TRAV26-1*01 5'-TACAGGAAGCCTCAGCA GATGCTAAGACCACCCAGCCCCC-3' TRAV26-1*02 5'-TACAGGAAGCCTCAGCA GATGCTAAGACCACCCAGCCCACC-3' TRAV26-2*01 5'-TACAGGAAGCCTCAGCA GATGCTAAGACCACACAGCCAAATTCAATG-3' TRAV27*01 5'-TACAGGAAGCCTCAGCA ACCCAGCTGCTGGAGCAGAGCC-3' TRAV29/DV5*01 5'-TACAGGAAGCCTCAGCA GACCAGCAAGTTAAGCAAAATTCACCATC-3' TRAV30*01 5'-TACAGGAAGCCTCAGCA CAACAACCAGTGCAGAGTCCTCAAGC-3' TRAV34*01 5'-TACAGGAAGCCTCAGCA AGCCAAGAACTGGAGCAGAGTCCTCAG-3' TRAV35*01 5'-TACAGGAAGCCTCAGCA GGTCAACAGCTGAATCAGAGTCCTCAATC-3' TRAV36/DV7*01 5'-TACAGGAAGCCTCAGCA GAAGACAAGGTGGTACAAAGCCCTCTATCTC-3' TRAV36/DV7*02 5'-TACAGGAAGCCTCAGCA GAAGACAAGGTGGTACAAAGCCCTCAATC-3' TRAV38-1*01 5'-TACAGGAAGCCTCAGCA GCCCAGACAGTCACTCAGTCTCAACCAG-3' TRAV38-1*04 5'-TACAGGAAGCCTCAGCA GCCCAGACAGTCACTCAGTCCCAGC-3' TRAV38-2/DV8*01 5'-TACAGGAAGCCTCAGCA GCTCAGACAGTCACTCAGTCTCAACCAGAG-3' TRAV39*01 5'-TACAGGAAGCCTCAGCA GAGCTGAAAGTGGAACAAAACCCTCTGTTC-3' TRAV40*01 5'-TACAGGAAGCCTCAGCA AGCAATTCAGTCAAGCAGACGGGC-3' TRAV41*01 5'-TACAGGAAGCCTCAGCA AAAAATGAAGTGGAGCAGAGTCCTCAGAAC-3' Supplementary Table 3: Vβ-gene-specific primers for cloning TCRβ genes TRBV gene β ID Vβ TRBV1*01 5'-CAGGAGGGCTCGGCA GATACTGGAATTACCCAGACACCAAAATACCTG-3' TRBV2*01 5'-CAGGAGGGCTCGGCA GAACCTGAAGTCACCCAGACTCCCAG-3' TRBV3-1*01 5'-CAGGAGGGCTCGGCA GACACAGCTGTTTCCCAGACTCCAAAATAC-3' TRBV3-2*01 5'-CAGGAGGGCTCGGCA GACACAGCCGTTTCCCAGACTCCA-3' TRBV4-1*01 5'-CAGGAGGGCTCGGCA GACACTGAAGTTACCCAGACACCAAAACAC-3' TRBV4-1*02 5'-CAGGAGGGCTCGGCA CACCTGGTCATGGGAATGACAAATAAGAAG-3' TRBV4-2*01 5'-CAGGAGGGCTCGGCA GAAACGGGAGTTACGCAGACACCAAG-3' TRBV4-3*04 5'-CAGGAGGGCTCGGCA AAGAAGTCTTTGAAATGTGAACAACATCTGGG-3' TRBV5-1*01 5'-CAGGAGGGCTCGGCA AAGGCTGGAGTCACTCAAACTCCAAGATATC-3' TRBV5-1*02 5'-CAGGAGGGCTCGGCA AGGGCTGGGGTCACTCAAACTCC-3' TRBV5-3*01 5'-CAGGAGGGCTCGGCA GAGGCTGGAGTCACCCAAAGTCCC-3' TRBV5-4*01 5'-CAGGAGGGCTCGGCA GAGACTGGAGTCACCCAAAGTCCCAC-3' TRBV5-4*03 5'-CAGGAGGGCTCGGCA CAGCAAGTGACACTGAGATGCTCTTCTCAG-3' TRBV5-4*04 5'-CAGGAGGGCTCGGCA ACTGTGTCCTGGTACCAACAGGCCCT-3' TRBV5-5*01 5'-CAGGAGGGCTCGGCA GACGCTGGAGTCACCCAAAGTCC-3' TRBV5-8*01 5'-CAGGAGGGCTCGGCA GAGGCTGGAGTCACACAAAGTCCCAC-3' TRBV5-8*02 5'-CAGGAGGGCTCGGCA AGGACAGCAAGCGACTCTGAGATGC-3' TRBV6-1*01 5'-CAGGAGGGCTCGGCA AATGCTGGTGTCACTCAGACCCCA-3' TRBV6-4*01 5'-CAGGAGGGCTCGGCA ATTGCTGGGATCACCCAGGCAC-3' TRBV6-4*02 5'-CAGGAGGGCTCGGCA ACTGCTGGGATCACCCAGGCAC-3' TRBV7-1*01 5'-CAGGAGGGCTCGGCA GGTGCTGGAGTCTCCCAGTCCCTG-3' TRBV7-2*01 5'-CAGGAGGGCTCGGCA GGAGCTGGAGTCTCCCAGTCCCC-3' TRBV7-2*04 5'-CAGGAGGGCTCGGCA GGAGCTGGAGTTTCCCAGTCCCC-3' TRBV7-3*01 5'-CAGGAGGGCTCGGCA GGTGCTGGAGTCTCCCAGACCC-3' TRBV7-3*05 5'-CAGGAGGGCTCGGCA TGGGAGCTCAGGTGTGATCCAATTTC-3' TRBV7-4*01 5'-CAGGAGGGCTCGGCA GGTGCTGGAGTCTCCCAGTCCC-3' TRBV7-6*01 5'-CAGGAGGGCTCGGCA GGTGCTGGAGTCTCCCAGTCTCCC-3' TRBV7-9*01 5'-CAGGAGGGCTCGGCA GATACTGGAGTCTCCCAGAACCCCAG-3' TRBV7-9*03 5'-CAGGAGGGCTCGGCA GATACTGGAGTCTCCCAGGACCCCAG-3' TRBV7-9*04 5'-CAGGAGGGCTCGGCA ATATCTGGAGTCTCCCACAACCCCAGAC-3' TRBV7-9*07 5'-CAGGAGGGCTCGGCA CACAACCGCCTTTATTGGTACCGACAG-3' TRBV9*01 5'-CAGGAGGGCTCGGCA GATTCTGGAGTCACACAAACCCCAAAGC-3' TRBV10-1*01 5'-CAGGAGGGCTCGGCA GATGCTGAAATCACCCAGAGCCCAAG-3' TRBV10-2*01 5'-CAGGAGGGCTCGGCA GATGCTGGAATCACCCAGAGCCCA-3' TRBV10-2*02 5'-CAGGAGGGCTCGGCA AAGGCAGGTGACCTTGATGTGTCACC-3'

TRBV11-1*01 5'-CAGGAGGGCTCGGCA GAAGCTGAAGTTGCCCAGTCCCC-3' TRBV11-2*01 5'-CAGGAGGGCTCGGCA GAAGCTGGAGTTGCCCAGTCTCCCAG-3' TRBV11-3*01 5'-CAGGAGGGCTCGGCA GAAGCTGGAGTGGTTCAGTCTCCCAGA-3' TRBV11-3*03 5'-CAGGAGGGCTCGGCA GGTCTCCCAGATATAAGATTATAGAGAAGAAACAGC-3' TRBV12-1*01 5'-CAGGAGGGCTCGGCA GATGCTGGTGTTATCCAGTCACCCAGG-3' TRBV12-2*01 5'-CAGGAGGGCTCGGCA GATGCTGGCATTATCCAGTCACCCAAG-3' TRBV12-3*01 5'-CAGGAGGGCTCGGCA GATGCTGGAGTTATCCAGTCACCCC-3' TRBV12-5*01 5'-CAGGAGGGCTCGGCA GATGCTAGAGTCACCCAGACACCAAGG-3' TRBV13*01 5'-CAGGAGGGCTCGGCA GCTGCTGGAGTCATCCAGTCCCC-3' TRBV14*01 5'-CAGGAGGGCTCGGCA GAAGCTGGAGTTACTCAGTTCCCCAGC-3' TRBV15*01 5'-CAGGAGGGCTCGGCA GATGCCATGGTCATCCAGAACCCAAG-3' TRBV16*01 5'-CAGGAGGGCTCGGCA GGTGAAGAAGTCGCCCAGACTCCA-3' TRBV17*01 5'-CAGGAGGGCTCGGCA GAGCCTGGAGTCAGCCAGACCC-3' TRBV18*01 5'-CAGGAGGGCTCGGCA AATGCCGGCGTCATGCAGAAC-3' TRBV19*01 5'-CAGGAGGGCTCGGCA GATGGTGGAATCACTCAGTCCCCAAAG-3' TRBV20-1*01 5'-CAGGAGGGCTCGGCA GGTGCTGTCGTCTCTCAACATCCGAG-3' TRBV20/OR9-2*01 5'-CAGGAGGGCTCGGCA AGTGCTGTCGTCTCTCAACATCCGAG-3' TRBV21-1*01 5'-CAGGAGGGCTCGGCA GACACCAAGGTCACCCAGAGACCTAGAC-3' TRBV21/OR9-2*01 5'-CAGGAGGGCTCGGCA GACACCAAGGTCACCCAGAGACCTAGATTTC-3' TRBV23-1*01 5'-CAGGAGGGCTCGGCA CATGCCAAAGTCACACAGACTCCAGG-3' TRBV24-1*01 5'-CAGGAGGGCTCGGCA GATGCTGATGTTACCCAGACCCCAAG-3' TRBV25-1*01 5'-CAGGAGGGCTCGGCA GAAGCTGACATCTACCAGACCCCAAGATAC-3' TRBV26*01 5'-CAGGAGGGCTCGGCA GATGCTGTAGTTACACAATTCCCAAGACACAG-3' TRBV26/OR9-2*01 5'-CAGGAGGGCTCGGCA GATGCTGTAGTTACACAATTCTCAAGACACAGAATC-3' TRBV27*01 5'-CAGGAGGGCTCGGCA GAAGCCCAAGTGACCCAGAACCC-3' TRBV28*01 5'-CAGGAGGGCTCGGCA GATGTGAAAGTAACCCAGAGCTCGAGATATC-3' TRBV29-1*01 5'-CAGGAGGGCTCGGCA AGTGCTGTCATCTCTCAAAAGCCAAGC-3' TRBV29-1*03 5'-CAGGAGGGCTCGGCA ACGATCCAGTGTCAAGTCGATAGCCAAG-3' TRBV30*01 5'-CAGGAGGGCTCGGCA TCTCAGACTATTCATCAATGGCCAGCG-3' TRBV30*04 5'-CAGGAGGGCTCGGCA ACTATTCATCAATGGCCAGCGACCC-3' Supplementary Table 4: Primers for Enrichment PCR Name PS1 partial PS2-α ID PS2-β ID DNA Sequence 5 -TCGTCGGCAGCGTCAGATG-3 5 -GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTACAGGAAGCCTCAGCA-3 5 -GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCAGGAGGGCTCGGCA-3 Supplementary Table 5. Primers for Adapter Insertion PCR Name DNA Sequence PS3-PS1 5 - AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTCAGATGTGTATAAGA GACAG-3 PS4-PS2 5 - CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGGAGATGTGTATAAGAGAC AG-3

Supplementary Table 6. Donor 1 TCR α and β genes and CDR3 amino acid sequences. CDR3 Gene Run 1 CASSTVSGAPSEQFF TRBV6-5*00 CALYFFFGNEKLTF TRAV16*00 CLE*IME_SQGNLIF TRAV4*00 CAVEYGNQFYF TRAV3*00 Run 2 CLE*IME_SQGNLIF TRAV4*00 CAVEYGNQFYF TRAV3*00 CASSLKT_NYEQYF TRBV12-4*00 CASNTGNQFYF TRAV24*00 CALYFFFGNEKLTF TRAV16*00 CLE*IME_S*GNLIF TRBV6-5*00 Supplementary Table 7. Donor 2 TCR α and β genes and CDR3 amino acid sequences from FACS. α CDR3 Gene β CDR3 Gene α-1 CAFMTSG~ATNKLIF TRAV38-1 β-1 CSVWSFGDRDGYTF TRBV29-1 α-2 CAVNDRGSTLGRLYF TRAV12-2 β-2 CASSSANYGYTF TRBV12-3 α-3 CAVNFGGGKLIF TRAV12-2 β-3 CASSSSPGLDNEQFF TRBV7-6 α-4 CPREEGGSQGNLIF TRAV26-1 β-4 CASSYITGTGSYGYTF TRBV6-5 α-5 CAAAVETSGSRLTF TRAV21 β-5 CSARDRIGNTIYF TRBV20-1 α-6 CARNTGNQFYF TRAV24 α-7 CTSPNTNAGKSTF TRAV26-1 Supplementary Table 8. Donor 2 TCR α and β genes and CDR3 amino acid sequences from MATE-seq. α CDR3 Gene FACS? β CDR3 Gene FACS? CPREEGGSQGNLIF TRAV26-1 α-4 CASSSSPGLDNEQFF TRBV7-6 β-3 CKASKIIF TRAV3 CASSEGSWGSDTQYF TRBV6-5 CAVRGDYKLSF TRAV1-2 CASSLGAGPYNEQFF TRBV18 CAVNDRGSTLGRLYF TRAV12-2 α-2 CASSGPISSYNEQFF TRBV6-1 CIAQNNNDMRF TRAV26-2 CAWSVSDLAKNIQYF TRBV30 CASSLSFGTEAFF TRBV6-4 CASSYPGTGIHGYTF TRBV6-5 CASSLGPSSYEQYF TRBV5-1 CASSEQAGGYGYTF TRBV6-1

Supplementary Movie 1 shows the removal of free pnp to waste and displacement of barcoded cells to the droplet generator, where individual cells are encapsulated in water-in-oil droplets with lysis RT-PCR mix. Supplementary Movie 2 shows a fluorescent sequence of viability-stained cells at the output of a DLD array with circular posts. Supplementary Movie 3 shows a fluorescent sequence of viability-stained cells at the output of a DLD array with I-posts.