Supplemental Data. Antony et al. (2010). Plant Cell /tpc

Transkript

1 Sb05g MAG--LSLQHPMAFAFGLLGNIISFMTYLAPLYRPTFYRIYKSKSTQGFQSVPYVVALF 57 Zm MAG--LSLQHPMAFAFGLLGNIISFMTYLAPL--PTFCRIYRNKSTEGFQSVPYVVALF 55 Zm MAG--LSLLHPMAFAFGLLGNIISFMTYLAPL--PTFYRIYKNKSTEGFQSVPYVVALF 55 11N3 MAG--MSLQHPWAFAFGLLGNIISFMTYLAPL--PTFYRIYKSKSTQGFQSVPYVVALF 55 Sb08g MAG--LSLQHPWAFAFGLLGNVISFLTFLAPI--PTFYRIYKSKSTEGFQSVPYVVALF 55 Sb08g MAG--LSLQHPWAFAFGLLGNVISFMTFLAPI--PTFYRIYKTKSTEGFQSVPYVVALF 55 Zm MAG--MSLQHPWAFAFGLLGNVISFMTFLAPI--PTFYRIYKSKSTEGFQSVPYVVALF 55 Zm MAG--LSLEHPWAFAFGLLGNVISFMTFLAPI--PTFYRIYKSKSTEGFQSVPYVVALF 55 Sb08g MAG--LSLQHPWAFAFGLLGNLISFLTFLAPI--PTFYRIYKTKSTEGFQSVPYVVALF 55 Zm MAG--LSLQHPWAFTFGLLGNVISFMTFLAPI--PTFYRIYKSKSTEGFQSVPYVVALF 55 Os12g MAG--LSLQHPWAFAFGLLGNLISFTTYLAPI--PTFYRIYKSKSTEGFQSVPYVVALF 55 UPA16 MTG--ISGH--WAFAFGVLGNIISFIVFLSPI--PTFYTIYKKKTAEGYQSIPYVIALF 53 At5g23660 MAL--FDTHNTWAFVFGLLGNLISFAVFLSPV--PTFYRICKKKTTEGFQSIPYVVALF 55 GmABT17358 MA----INHETWAFVFGLLGNVISFMVFLAPL--PTFYQIYKKKSTEEFQSLPYVVALF 53 Sb04g MAFL-NMEQQTWAFTFGILGNIISLMVFLSPL--PTFYRVYRKKSTEGFQSTPYVVTLF 56 Zm MAFL-NMEQQTWAFTFGILGNIVSLMVFLSPL--PTFYRVYRNKSTEGFQSTPYVVTLF 56 Os02g MAFM-SMERSTWAFTFGILGNLISLMVFLSPL--PTFYRVYRKKSTEGFQSTPYVVTLF 56 Zm MAGGLFSMAHPAVTLSGIAGNIISFLVFLAPV--ATFLQVYRKKSTGGFSSVPYVVALF 57 Sb07g MAGGLFSMAHPAITLSGIAGNIISFLVFLAPV--ATFLQVYRKKSTGGFSSVPYVVALF 57 8N3 MAGGFLSMANPAVTLSGVAGNIISFLVFLAPV--ATFLQVYKKKSTGGYSSVPYVVALF 57 Sb02g MAGGLFSMEHPWVSAFGILGNIISFLVFLAPV--PTFLRVYRKKSTEGFSSVPYVVALF 57 Zm MAGGLFSMEHPWASVFGILGNIISFLVFLAPV--PTFLRVYRKKSTEWFSSVPYVVALF 57 Sb05g SAMLWIYYALLKSNE-FLLITINSAGCVIETLYIVMYLLYAPKKAKLFTAKILLLLNVGV 116 Zm SAMLWIYYALLKSNE-FLLITINSAGCVIETLYIATYLLYAPNKAKLFTAKILLLLNVGV 114 Zm SAMLWIYYALLKSNE-LLLITINSAGCVIETLYIAMYLLYAPKKAKLFTAKILLLLNVGV N3 SAMLWIYYALLKSDE-CLLITINSAGCVIETIYIAVYLVYAPKKAKMFTAKLLLLVNVGV 114 Sb08g SAMLWIFYALIKSNE-TFLITINAAGCVIETIYIVMYFVYAPKKAKLFTAKIMLLLNVGV 114 Sb08g SAMLWIFYALIKSNE-TFLITINAAGCVIETIYIIMYFVYAPKKGKMFTAKIMLLLNVGI 114 Zm SAMLWIFYALIKSNE-TFLITINAAGCVIETIYVVMYFVYAPKKAKLFTAKIMVLLNGGV 114 Zm SAMLWIFYALIKSNE-TFLITINAAGCVIETIYIVMYFVYAPKKAKLFTAKIMALLNGGV 114 Sb08g SAMLWIFYALIKSNE-TFLITINAAGCVIETIYIVMYFVYAPKKAKLFTAKIMLLLNVGV 114 Zm SAMLWIFYALIKSNE-TFLITINAAGCVIETVYVVMYFVYATKKGRMFTAKIMLLLNVGA 114 Os12g SAMLWIFYALIKSNE-ALLITINAAGCVIETIYIVMYLAYAPKKAKVFTTKILLLLNVGV 114 UPA16 SSMLWIYYAFLKTNV-TLLITINSFGIFIETIYVGLYLFYAPKKARVHTVKMLLLTVVGG 112 At5g23660 SAMLWLYYATQKKDV-FLLVTINSFGCFIETIYISIFVAFASKKARMLTVKLLLLMNFGG 114 GmABT17358 SSMLWIYYALVKKDASLLLITINSFGCVIETIYLAIFLIYAPSKTRLWTIKLLLMLNVFG 113 Sb04g SCMLWIFYALLKSGA-ELLVTINGVGCVIETVYLGMYLLYAPKAARVLTAKMLLGLNVGV 115 Zm SCMLWILYALLKPGA-ELLVTINGVGCVVETVYLAMYLVYAPKAARVLAAKMLLGLNVAV 115 Os02g SCMLWMYYAFVKSGA-ELLVTINGVGCVIETVYLAMYLAYAPKSARMLTAKMLLGLNIGL 115 Zm SSVLWIFYALVKTNS-RPLLTINAFGCGVEAAYIVLYLAYAPRRARLRTLAYFFLLDVAA 116 Sb07g SSVLWIFYALVKTNS-RPLLTINAFGCGVEAAYIVFYLAYAPRKARLRTLAYFFLLDVAA 116 8N3 SSVLWIFYALVKTNS-RPLLTINAFGCGVEAAYIVLYLVYAPRRARLRTLAFFLLLDVAA 116 Sb02g SCTLWILYAVVKTNS-SPLLTINAFGCVVEATYILLYLIYAPRAARLRALAFFFLLDVAA 116 Zm SCTLWILYALVKTNS-SPLLTINAFGCVVEAAYILLYLVYAPRGARLRALASFLLLDVAA 116 Sb05g FGLILLLTLLLSAGQHRVVVLGWVCVAFSVSVFVAPLSIIRQVVRTRSVEFMPFSLSLSL 176 Zm FGLILLLTLLLSAGPHRVVVLGWVCVAFSVSVFVAPLSIIRQVVRTRSVEFMPFSLSFSL 174 Zm FGLILLLTLLLSAGQRRVVVLGWVCVAFSVSVFVAPLSIIRQVVRTRSVEFMPFSLSLSL N3 FGLILLLTLLLSAGDRRIVVLGWVCVGFSVSVFVAPLSIIRLVVRTKSVEFMPFSLSFSL 174 Sb08g FGVILLVTLLLFKGDKRVVMLGWICVGFSVSVFVAPLSIMRRVIQTKSVEYMPFSLSLSL 174 Sb08g FGVILLLTLLLFKGDKRVVMLGWICVGFSVSVFVAPLSIMKRVIQTKSVEYMPFSLSLSL 174 Zm FGVILLLTLLLFKGSKRVVLLGWICVGFSVSVFVAPLSIMRRVIQTKSVEYMPFSLSLSL 174 Zm FGVILLLTLLLFKGSKRVVLLGWICVGFSVSVFVAPLSIMRRVIQTKSVEYMPFSLSLSL 174 Sb08g FGVILLVTLLLFKGDKRVVMLGWICVGFSVSVFVAPLSIMRRVIQTKSMEYMPFSLSLSL 174 Zm FGAILLLTLLLFKGDKRVVMLGWICVGFSVSVFVAPLSIMRRVIQTKSVEYMPFSLSLSL 174 Os12g FGVILLLTLLLSHGEQRVVSLGWVCVAFSVSVFVAPLSIIKRVIQSRSVEYMPFSLSLTL 174 UPA16 FGAIVLVTQFLFKGVVRGQIVGWICLIFALSVFVAPLGIVRQVIKTKSVEYMPLLLSVFL 172 At5g23660 FCLILLLCQFLAKGTTRAKIIGGICVGFSVCVFAAPLSIIRTVIKTKSVEYMPFSLSLTL 174 GmABT17358 FGAMLLSTLYLTTGSKRLTVIGWICLVFNISVFAAPLCIIKRVIKTKSVEFMPFSLSFFL 173 Sb04g FGLVALVTMVLSNGGLRVKVLGWICVSVALSVFAAPLSIMRQVIRTKSVEFMPISLSFFL 175 Zm FGLVALVTMLLSDAGLRVHVLGWICVSVSLSVFAAPLSIMRQVIRTKSVEFMPISLSFFL 175 Os02g FGVIALVTLLLSRGELRVHVLGWICVAVSLSVFAAPLSIIRLVIRTKSVEFMPFSLSFFL 175 Zm FALVVAVTLFAVREPHRVKFLGSVCLAFSMAVFVAPLSIIVKVVKTKSVEFLPISLSFCL 176 Sb07g FALVVVVTLFVVREPHRVKFLGSVCLAFSMAVFVAPLSIIVKVVKTKSVEFLPISLSFCL 176 8N3 FALIVVTTLYLVPKPHQVKFLGSVCLAFSMAVFVAPLSIIFKVIKTKSVEFMPIGLSVCL 176 Sb02g LALIVVVVVVLVAEPHRVKVLGSICLAFSMAVFVAPLSVIFVVIRTKSAEFMPFTLSFFL 176 Zm FSLVAVVTVVLVAEPHRVRVLGSVCLAFSMAVFVAPLSVIFVVIRTKSAEFMPFTLSFFL 176 Sb05g TVSAVVWFLYGLLIKDKYVALPNVLGFSFGVVQMGLYALYRNATPRVPPA Zm TASAVVWFLYGLLIKDKYVALPNVLGFTFGVVQMGMYALYRNATPRVPAA Zm TVSAVVWFLYGLLIKDKYVALPNVIGFSFGVVQMGLYALYRNATPRVP-A N3 TISAVVWFLYGLLIKDKYVALPNVLGFSFGVIQMGLYAMYRNSTPKAVLT Sb08g TLSAVVWFLYGLLIKDKYVALPNILGFTFGVVQMVLYVLYMNKTPVAV-A Sb08g TLSAVVWFLYGLLIKDKYVALPNILGFTFGVVQMVLYVLYMNKTPVAV-A Zm TLSAVVWFLYGLLIKDKYVALPNILGFTFGVVQMVLYVLYMNKTPVAATA Zm TLSAVVWFLYGLLIKDKYVALPNVLGFIFGVVQMVLYVFYMNKTPVAA-A Sb08g TLSAVVWFLYGLLIKDKYVALPNILGFTFGMVQMVLYVLYMNKTPVAV-A Zm TLSAVVWFLYGLLIKDKYVALPNILGFTFGVVQMVLYVVYMNKTPLPV-A Os12g TLSAVVWFLYGLLIKDKYVALPNILGFTFGVVQMGLYVFYMNATPVAG UPA16 TLSAVMWFFYGLLLKDINIAAPNVLGFIFGVLQIVLYAIYSKKEKVILKE At5g23660 TISAVIWLLYGLALKDIYVAFPNVIGFVLGALQMILYVVYKY GmABT17358 TINAVMWFFYGLLLKDYYVALPNTLGFLFSIIQMVLYLIYRN Sb04g VLSAVIWFAYGALKKDVFVAAPNVLGFVFGLAQMALYMAY-RNKKPAAAA Zm VLSAVVWFAYGALKKDVFVAFPNVLGFVFGLAQMALYMAYSRNRKPAAAL Os02g VLSAVIWFLYGLLKKDVFVALPNVLGFVFGVAQMALYMAY-RSKKPLVASSSSAVVAAGL 234 Zm TLSAVAWFCYGLFTKDPFVMYPNVGGFFFSCVQMGLYFWYRKPRPAAK Sb07g TLSAVAWFCYGLFTKDPFVMYPNVGGFFFSCVQMGLYFWYRKPRP-AK N3 TLSAVAWFCYGLFTKDPYVMYPNVGGFFFSCVQMGLYFWYRKPRN Sb02g TLSAVAWFLYGIFTKDPYVTLPNVGGFFFGCIQMVLYCCYRK-PS Zm TLSAVAWFLYGLFTKDPYVTLPNVGGFFFGCIQMVLYCCYRKRKP

2 Sb05g KEVTDDDAAADG TFKLPGEH-VVTIAKLT AVPAVS 260 Zm KEAAA--AADDGNT------FNFKAPGEH-VVTIAKLTA AAPATA 260 Zm KDVADD-ASKD KAPGEHVVVTIAKLTA ATTAPA N KEVEAATATGDDD HSAAGVKEH-VVNIAKLSA AVDVVK 261 Sb08g EGKDAGVKLPSAA DEHVLVNITKLSP ALPDRS 255 Sb08g EGKDAGGKLPSAA DEHVLVNIAKLSP ALPERS 255 Zm EGKDAG-KLSSAA DEHVLVNIAKLSP ALPERS 255 Zm VGKDAG-KLPSAA DEHVLVNIAKLNP ALPERT 254 Sb08g EGKDAGGKLPSAG DKHVLVNIAKLSP ALPERS 255 Zm DGKAAG-KLPSAA DEHVVVNVTKLSPG RLP Os12g EGKEGKGKLAAAE ELPVVVNVGKLAA ATPDRS 254 UPA16 --QKLPEIQKPAVIVAD DNTNANKKLPELTHEQI IDIVK 259 At5g CKTPSDLVEK ELEAA--KLPEVS IDMVK 242 GmABT AKTP DLPMKLQELNSHT IDVGK 237 Sb04g VIMVEEVKLPAEQYASKEVAPPAAAHEGSRASCG-AEVHPIDIDTLPVADVGRHHDSQA 282 Zm VILPEQSKE EAAEG-KASCGGAEVHPIDIAEV HDLQA 261 Os02g EIKLPEHVKEVQAVAKG----AVAAAPEG-RISCG-AEVHPID DVMP 275 Zm NNAVLPTTTDGGN AVQVQGQVIELAP NTVAILSV 258 Sb07g NNAVLPTTTDGAS AVQMQGQVIELAP NTVAILSV 257 8N TAVLPTTSDSMSPIS AAAAATQRVIELPAGT------HAFTILSV 260 Sb02g ASVVLPTTTDAAAT EMELPL AAHQ Zm ASVVLPTTTAAAAVA QQLEAEMELPL AAHQHQ 253 Sb05g P----QLQEEAK------PADNGTTP APAPANDVQLN--AEQV Zm A----ELIIKARDDAQHPPEEEAAAA KAAPAKSKLLIPLPEHAYACMCIIRSG 309 Zm A----AVAEDLVKVHDG-HPEEAAKG AAKPAENGAGRSDAEQV Os11N3 TRE--VHPVDVESPPAEAPPEEDDKA AAATAAAVAGAGEKKVAA Sb08g SGVH-RATQMAAVPASSCAAEAAAP AMLPNRDVVDVFVSRQSPAVHVV Sb08g SGVHPVVAQMAAVPNRSCAAEAAAPP AMLPNRDVVDVFVSRHSPAVHVV Zm SGVH-PVTQMAGVPVRSCAAEATAP AMLPNRDVVDVFVSRHSPAVHVA Zm SGMH-PVTQMAAVPARSCAAEAIAP AMLPNRDVVDVFVSRHSPAVHVV Sb08g SGVH-RATQMSAVPAKSCAAEATAPK VMLPNRDVVDVFLSQALHRKQA Zm PVTQMAAVPTKSCATEAAAP ATLPSRDVVDVLVNRHSPAVHVT Os12g TG----AVHVHPVP-RSCAAEAAAA EPEVLVDIPPPPPPRAVEVAAV CaUPA16 LAGLLTCTEKSHVATCP--HDVNCGV EATNVENNIPKLQTVEAT At5g23660 LG-TLTSPEPVAITVVRSVNTCNCND RNAEIENGQGVRNSAATT GmABT17358 LS-RMEPSEPNHVTKNG TLTEREI Sb04g VVVIDVDVEPAATCAAAAAAAGGVRGDGAAGVVTAGPEQPAAMKPVDMAIAVEA Zm VVV-DVDVEPVTYAAASGMVDGSVGR PRAPEQLVIKPDMVTVIAAEA Os02g SEVVEVKVDDEETNRTDEMAGDGDHA MVRTEQIIKPDMAIVVEV Zm SPIPIVGVHKIEVVEQQHKEAAVAAE TRRMAAANPDGAMPEVIEIVPAAAAV- 310 Sb07g SPIPIVGVHKIEVVEQQHKEAAVAAE TRRMAAANPDGAMPEVIEIVPAVATV- 309 Os8N3 SPIPILGVHKVEVVAAEQAADGVAAA AAADKELLQN--KPEVIEITAAV Sb02g AVAPVLAELQKLEEAMGS PRKHGG VVKVV Zm LAVAVLPTCAAPVLAELQKLEEAMGS PRKGG VKAI Sb05g Zm SHHKLGRACLLGTSTRPPACLPARMIQSSCYIRKG 344 Zm Os11N Sb08g Sb08g Zm Zm Sb08g Zm Os12g CaUPA At5g GmABT Sb04g Zm Os02g Zm Sb07g Os8N Sb02g Zm Supplemental Figure 1. ClustalW alignment of N3 proteins.

3 ATCTCCT 11a2-5 TTGATCTCCT 11a2-4 ATCTCTGCTTTATTGCCTGATCATCCT 11a2-14 AAGCCTTCAAGCAAAGCAAACTCAAGTAGTAGCTGATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a2-6 AAGCCTTCAAGCAAAGCAAACTCAAGTAGTAGCTGATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a2-8 AAGCCTTCGAGCGCTGAAAATAA-AGTAGTAGCTGATTACCAGCTCTTCTCTCTTCTGATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a ATCAAGCCTTCAAGCAAAGCAAACTCAAGTAGTAGCTGATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT OS11N3 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-2 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-4 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-5 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-6 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-11 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-14 GATTACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-15 ACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-1 ACCAGCTCTTCTCTCTTCTCATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-12 GAGTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-3 ATTGAGAAGAGGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-8 GGGAATTAAGTTTTGATCTCTGCTTTATTGCCTGATCATCCT 11a7-7 ATCTCTGCTTTATTGCCTGATCATCCT 11a7-13 Supplemental Figure 2. Alignment of Os-11N3 transcription sites after inoculation with ME2 or ME2 (avrxa7). 5 -RACE cdnas were derived from leaf tissue of cultivar Nipponbare 24 h after inoculation with ME2 or ME2(avrXa7) are aligned above and below, respectively, to the predicted 5 -UTR of Ds-11N3 (letters in bold). Numbering starts at base 28 downstream of the TATA box.

4 Supplemental Table 1. Strains and plasmids used in the study. Strain or plasmid Relevant characteristics Reference/Source Strains Xanthomonas oryzae pv. oryzae PXO99 A 5-Azacytidine resistant, race 6 1 PXO99 A ME7 hrpc mutant, Kn r 2 PXO99 A ME2 pthxo1 mutant of PXO99 A 3 ME2 (pthxo1) ME2 with pzwpthxo1 in phm1 3 ME2 (avrxa7) ME2 with pzwavrxa7 in phm1 3 ME2 (pthxo2) ME2 with pzwpthxo2 in phm1 3 ME2 (pthxo3) ME2 with pzwpthxo3 in phm1 3 ME2(avrXa7-2F) ME2(pthXo1-2F) ME2 with pzwavrxa7-2f in phm1 with two FLAG epitopes sequences incorporated into C-terminal coding region. Sequences available upon request. ME2 with pzwpthxo1-2f in phm1 with two FLAG epitopes sequences incorporated into C-terminal coding region. Sequences available upon request. This study This study Plasmids pbluescript II KS(+) Phagemid, puc derivative, Cb r Stratagene phm1 Broad host range plasmid, Sp r 1 pentr/d-topo Phagemid, Cb r, Kn r Invitrogen p35s-pthxo1 pthxo1 under CaMV 35S promoter in pcambia1300 This study p35s-avrxa7 avrxa7 under CaMV 35S promoter in pcambia1300 This study panda Gateway RNAi vector, Kn r 4 pcambia1300 Binary T-DNA vector for rice transformation Cambia pbi121 T_DNA vector 5 1. Hopkins C.M., White F.F., Choi S.-H., Guo A., and Leach J.E. (1992). Identification of a family of avirulence genes from Xanthomonas oryzae pv. oryzae. Mol. Plant-Microbe Interact. 5: Zhu W., Magbangua M.M., and White FF. (2000). Identification of two novel hrp-associated genes in the hrp gene cluster of Xanthomonas oryzae pv. oryzae. J. Bacteriol. 182, Yang B. and White FF Diverse members of AvrBs3/PthA family of type III effectors are major virulence determinants in bacterial blight of rice. Mol. Plant-Microbe Interact. 17, Miki D. and Shimamoto K. (2004). Simple RNAi vectors for stable and transient suppression of gene function in rice. Plant Cell Physiol. 45, Jefferson RA, Kavanagh T.A., and Bevan M.W. (1987). GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6,

5 Supplemental Table 2. Primers used in this study. RT-8N3-F 5 GACTCCATGTCCCCGATCTCC-3 RT-8N3-R 5 CACCACCTCGACCTTGTGCA-3 11N3RNAi-F 5 GAGAAGAAGGTAGCTGCATGAGTG-3 11N3RNAi-R 5 CAACCTGTAAGGGTTCCTTCCATGA-3 RT-11N3-F 5 ATCAAGCCTTCAAGCAAAGC-3 RT-11N3-R 5 CTAGGAGACCAAAGGCGAAG-3 RT-TF2-5F 5 -GGGTTTGCCTGGTATTTGTTAG-3 RT-TF2-5R 5 -GTTGCTGCTGTGATATACTCTG-3 Os11g-F 5 -GTCTCCTAGGTGTGTTGCCTTTG-3 Os11g-R 5 -GTGGGTGACGTCATGTTACACT RB-F 5 -CGTAACATAAGGGACTGACCTACC-3 8pG-F 5 -TACAAGCTTATATCAGAGTGAAAAAGAAATATCAAGC-3 8pG-R 5 -ATCTCTAGAACTAACTCTAAGGTGTTAATCAGTGAGAAGG-3 8pMGF 5 -TACAAGCTTATATCAGAGTGAAAAAGAAATATCAAGCACAAGAAAAAAAAGCAA AGGTTAGATATGGTGCTCCCC-3 11pG-F 5 TACAAGCTTCAGCTGGTCATGTGTGCCTTTTCATTCC-3 11pG-R 5 -ATCTCTAGACTAGCTCGGAATATGAGAGAGAGAGAGAGAGT-3 11pMG-F 5'-TACAAGCTTCAGCTGGTCATGTGTGCCTTTTCATTCCCTTCTTCCTTCCTAGC ACTATATAAACCGGTTCCAACC PG-F 5 -TACAAGCTTATATAAACCCCCTCCAACCAGGTGCTAACCAAAAGTGGAG-3 11M-8pG-F 5 -TACAAGCTTATATAAACCGGTTCCAACCAGGTGCTAACCAAAAGTGGAG-3 11N3-5 F 5 -GTCAGCAGCTGGTCATGTGT-3' 11N3-5 R 5 -GTTTGGTGGGAGGAGATCA-3 11N3-3 F 5 -AGGGACAAAACAAGACAGGTCA-3 11N3-3 R 5 GTGCACCACACCAGTTCTCT-3 8N3-5 F 5 -CAGAGTGAAAAAGAAATATCAAGC-3 8N3-5 R 5 -GGTGTTAATCAGTGAGAAGG-3 8N3-3 F 5'-GGAGAGTGCTGTATGGCGA-3 8N3-3 R 5 -GTCTGCCCTGTTCAAACACA-3