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TN HR TT b) i) 5-140 mg/l N 2,6-Dimethylphenole 284 Instrument specific information The test can be performed on the following devices. In addition, the required cuvette and the absorption range of the photometer are indicated. Instrument Type Cuvette λ Measuring Range SpectroDirect, XD 7000, XD 7500 b) i) ø 16 mm 340 nm 5-140 mg/l N Material Required material (partly optional): Reagents Packaging Unit Part Number Total Nitrogen 1 Set 2420703 The following accessories are required. Accessory Packaging Unit Part Number Measuring spoon No. 4 white PP 1 pc. 424515 Measuring spoon no. 8, black 1 pc. 424513 Application List Waste Water Treatment Drinking Water Treatment Raw Water Treatment Notes 1. This test determines the inorganic compounds Ammonia, Nitrate and Nitrite, as well as organic compounds like amino acid, urea, complexing agents etc. NL EN Handboek Handbook van of Methoden Methods 05/18

Digestion 0.5 ml 4.5 ml 16 mm Put 0.5 ml sample in the digestion vial. 16 mm Put 4.5 ml deionised water in the digestion vial. Add a level measuring scoop No. 8 (black) Digestion Reagent. Seal the vials in the preheated thermoreactor for 60 minutes at 100 C. 15 ºC 25 ºC Remove the vial from the thermoreactor. Note: vial will be hot!) Allow the vial(s) to cool to room temperature. NL EN Handboek Handbook van of Methods Methoden 05/18 05/18

Add a level measuring scoop No. 4 (white) Compensation Reagent. NL EN Handboek Handbook van of Methoden Methods 05/18

Implementation of the provision Nitrogen, total HR with Vial Test Select the method on the device For testing of Nitrogen, total HR with tube test, carry out the described digestion. For this method, no ZERO measurements are to be carried out with the following devices: XD 7000, XD 7500 Zero Place the supplied Zero vial (red sticker) in the sample chamber. Pay attention to the positioning. Press the ZERO button. For devices that require no ZERO measurement, start here. 0.5 ml Remove vial from the sample chamber. Sample Open a digestion vial. Fill sample vial with 0.5 ml prepared, digested sample. 0.2 ml Carefully invert several times to mix Note: Will get hot! 16 mm Add 0.2 ml Nitrate-111. NL EN Handboek Handbook van of Methods Methoden 05/18 05/18

Test 15 min Place sample vial in the sample chamber. Pay attention to the positioning. Press the TEST (XD: START) button. 10 11 12 1 2 9 3 8 7 6 5 4 Wait for 15 minute(s) reaction time. Once the reaction period is finished, the measurement takes place automatically. The result in mg/l Nitrogen appears on the display. NL EN Handboek Handbook van of Methoden Methods 05/18

Analyses The following table identifies the output values can be converted into other citation forms. Unit Cite form Scale Factor mg/l N 1 mg/l NH 4 1.288 mg/l NH 3 1.2158 Chemical Method 2,6-Dimethylphenole Appendix Interferences Persistant Interferences Nitrogen compounds which are hardly to oxidise, as may be found in industrial sewage, are not digested or only partially. According to US EPA 40 CFR 141 Derived from EN ISO 11905-1 a) determination of free, combined and total b) Reactor is necessary for COD (150 C), TOC (120 C) and total -chromium, - phosphate, -nitrogen, (100 C) c) MultiDirect: Adapter is necessary for Vacu-vials (Order code 19 20 75) d) Spectroquant is a Merck KGaA Trademark e) alternative reagent, used instead of DPD No.1/No.3 in case of turbidity in the water sample caused by high concentration of calcium and/or high conductivity f) additionally required for determination of bromine, chlorine dioxide and ozone in the presence of chlorine g) Reagent recovers most insoluble iron oxides without digestion h) additionally required for samples with hardness values above 300 mg/l CaCO 3 i) high range by dilution # including stirring rod, 10 cm NL EN Handboek Handbook van of Methods Methoden 05/18 05/18